| Cat. No. : |
HGF-226H |
| Product Overview : |
Recombinant Human HGF encoding the human HGF beta chain (Val495–Ser728) was recombined with his tag at the N-Terminus. That was expressed inE.Coli,and purified by Ni-NTA column. MW=26KD. |
| Description : |
Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. |
| Source : |
E. Coli. |
| Sequence : |
Human HGF beta chain (Val495–Ser728)495 VVNGIPTRTN IGWMVSLRYR NKHICGGSLI KESWVLTARQ CFPSRDLKDY EAWLGIHDVH GRGDEKCKQV LNVSQLVYGP EGSDLVLMKL ARPAVLDDFV STIDLPNYGC TIPEKTSCSV YGWGYTGLIN YDGLLRVAHL YIMGNEKCSQ HHRGKVTLNE SEICAGAEKI GSGPCEGDYG GPLVCEQHKM RMVLGVIVPG RGCAIPNRPG IFVRVAYYAK WIHKIILTYK VPQS 728. |
| Purity : |
|
| Formulation : |
Lyophilized 100 μg human HGF beta in 50 μl of TBS (20 mM Tris, 50mM NaCl, pH8.0). Carry free. |
| Application : |
Cell biology, ELISA. |
| Reconstitution : |
Add 500 μl deionized water to the vial to prepare a working stock solution at 200μg/mL. Allow to set at least 30 minutes at 4°C, mix well. |
| Storage : |
Store lyophilized protein at -20°C or -70°C. Lyophilized protein is stable for up to 6 months from date of receipt at -70°C. Upon reconstitution, this protein can be stored at for a few weeks or at -70°C in a manual defrost freezer for long term storage (six months). Aliquot reconstituted protein to avoid repeated freezing / thawing cycles. |
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