||P12 is a small subunit of the transcription factor IIA and has been shown to be required for both basal and activated transcription.Recombinant p12, along with two other subunits (α and β) can potentiate transcriptional activation, whereas p12 along with β-subunit is able to function in an antirepression. P12 subunit of TFIIA is isolated from a strain ofE. colithat contains the coding sequence for human TFIIA p12 under the control of T7 promoter.
||Recombinant p12 in combination with other subunits of TFIIA has been applied for in vitro transcription assays, gel mobility shift assays and protein-protein interaction assays.
||For in vitro use only.
||Liquid. Supplied in 20 mM Tris-HCl pH 8.0, 100 mM KCl, 0.2 mM EDTA, 1 mM DTT and 20% glycerol.
||1 ng of p12 is sufficient for a gel mobility shift assay, 10 ng are sufficient for transcription assay and 100 ng are sufficient for a protein-protein interaction assay.
||> 95% by SDS-PAGE.
||Quality guaranteed for 12 months, store at -80℃. Avoid freeze / thaw cycles.