"CKB" Related Products


Recombinant Human Creatine Kinase, Brain

Cat. No. : CKB-195H
Product Overview : Recombinant Human CKBBI produced inPichia Pastorisis a glycosylated polypeptide chain having an identical amino acid sequence compared to the native enzyme, purified under non-denaturing conditions and reacts with polyclonal antibodies to BB Isoenzyme in ELISA. The CKBBI is purified by proprietary chromatographic techniques.
Description : Creatine Kinase BB is a cytoplasmic enzyme involved in energy homeostasis. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. The encoded protein is a member of the ATP: guanido phosphotransferase protein family. A pseudogene of this gene has been characterized.
Source : Pichia Pastoris.
Physical Appearance : Sterile Filtered colourless liquid formulation.
Formulation : The protein (2.2 mg/ml) contains 10mM Bis-Tris-HCl pH-6.2, 50% glycerol, 0.5mM EDTA and 0.5mM DTT.
Stability : CKBBI although stable at 15°C for 7 days, should be stored desiccated below -18°C. Please prevent freeze-thaw cycles.
Purity : Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Biological Activity : The biological activity measured by the enzymatic activity of Creatine phosphokinase procedure No.45-UV, 1IU-1 µmole creatine phosphate was 500 IU/mg at 37°C.
Gene Name : CKB creatine kinase, brain [ Homo sapiens ]
Synonyms : CKB; creatine kinase, brain; B-CK; CKBB; creatine kinase-B; EC
Gene ID : 1152
mRNA Refseq : NM_001823
Protein Refseq : NP_001814
MIM : 123280
UniProt ID : P12277
Chromosome Location : 14q32.3
Pathway : Arginine and proline metabolism; Metabolic pathways; Metabolism of amino acids
Function : ATP binding; creatine kinase activity; nucleotide binding; protein binding

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