|Cat. No. :
|Product Overview :
||Recombinant Human CKBBI produced inPichia Pastorisis a glycosylated polypeptide chain having an identical amino acid sequence compared to the native enzyme, purified under non-denaturing conditions and reacts with polyclonal antibodies to BB Isoenzyme in ELISA. The CKBBI is purified by proprietary chromatographic techniques.
||Creatine Kinase BB is a cytoplasmic enzyme involved in energy homeostasis. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. The encoded protein is a member of the ATP: guanido phosphotransferase protein family. A pseudogene of this gene has been characterized.
|Physical Appearance :
||Sterile Filtered colourless liquid formulation.
||The protein (2.2 mg/ml) contains 10mM Bis-Tris-HCl pH-6.2, 50% glycerol, 0.5mM EDTA and 0.5mM DTT.
||CKBBI although stable at 15°C for 7 days, should be stored desiccated below -18°C. Please prevent freeze-thaw cycles.
||Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
|Biological Activity :
||The biological activity measured by the enzymatic activity of Creatine phosphokinase procedure No.45-UV, 1IU-1 µmole creatine phosphate was 500 IU/mg at 37°C.