Recombinant Human Creatine Kinase, Brain

• Cat.No.: CKB-195H
• Product Overview: Recombinant Human CKBBI produced inPichia Pastorisis a glycosylated polypeptide chain having an identical amino acid sequence compared to the native enzyme, purified under non-denaturing conditions and reacts with polyclonal antibodies to BB Isoenzyme in ELISA. The CKBBI is purified by proprietary chromatographic techniques.

Specification

• Cat. No.: CKB-195H
• Description: Creatine Kinase BB is a cytoplasmic enzyme involved in energy homeostasis. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. The encoded protein is a member of the ATP: guanido phosphotransferase protein family. A pseudogene of this gene has been characterized.
• Source: Pichia Pastoris.
• Physical Appearance: Sterile Filtered colourless liquid formulation.
• Formulation: The protein (2.2 mg/ml) contains 10mM Bis-Tris-HCl pH-6.2, 50% glycerol, 0.5mM EDTA and 0.5mM DTT.
• Stability: CKBBI although stable at 15°C for 7 days, should be stored desiccated below -18°C. Please prevent freeze-thaw cycles.
• Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Biological Activity: The biological activity measured by the enzymatic activity of Creatine phosphokinase procedure No.45-UV, 1IU-1 µmole creatine phosphate was 500 IU/mg at 37°C.

Gene Information

• Gene Name: CKB creatine kinase, brain [ Homo sapiens ]
• Synonyms: CKB; creatine kinase, brain; B-CK; CKBB; creatine kinase-B; EC 2.7.3.2
• Gene ID: 1152
• mRNA Refseq: NM_001823
• Protein Refseq: NP_001814
• MIM: 123280
• UniProt ID: P12277
• Chromosome Location: 14q32.3
• Pathway: Arginine and proline metabolism; Metabolic pathways; Metabolism of amino acids
• Function: ATP binding; creatine kinase activity; nucleotide binding; protein binding