Recombinant Human Dihydrofolate Reductase, His-tagged
Cat.No. : | DHFR-375H |
Product Overview : | Recombinant Dihydrofolate reductase protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography techniques. MW = 23.6 kDa (207aa). |
- Specification
- Gene Information
- Related Products
Cat. No. : | DHFR-375H |
Description : | DHFR, also known as Dihydrofolate reductase, is anenzymethat reducesdihydrofolic acidtotetrahydrofolic acid, usingNADPHaselectron donor, which can be converted to the kinds of tetrahydrofolatecofactorsused in 1-carbon transfer chemistry. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia. |
Source : | E.coli. |
Amino Acid Sequence : | MGSSHHHHHH SSGLVPRGSH MVGSLNCIVA VSQNMGIGKN GDLPWPPLRN EFRYFQRMTT TSSVEGKQNL VIMGKKTWFS IPEKNRPLKG RINLVLSREL KEPPQGAHFL SRSLDDALKL TEQPELANKV DMVWIVGGSS VYKEAMNHPG HLKLFVTRIM QDFESDTFFP EIDLEKYKLL PEYPGVLSDV QEEKGIKYKF EVYEKND |
Form : | Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 0.1M NaCl 2mM DTT, and 30% glycerol. |
Purity : | > 95% by SDS – PAGE. |
Concentration : | 1 mg/ml (determined by Bradford assay). |
Biological activity : | Specific activity is 1.5 - 2.5 units/ml and was obtained by measuring the oxidation of NADPH in absorbance at 340 nm during reaction. One unit will convert 1.0 umole of 7,8 dihydrofolate and beta-NADPH to 5,6,7,8-tetrahydrofolate and beta-NADP per minute at pH 6.5 at 25℃. |
Activity Assay : | 1.Prepare a 3.2 ml reaction mixture into a suitable container: The final concentrations are 50 mM potassium phosphate, 0.72 mM DHFA, 0.1mM beta-NADPH, 0.003% (w/v) BSA.2.Equilibrate to 25℃ and monitor the A340nm until the value is constant using a spectrophotometer.3.Add 2.5ug of recombinant DHFR into reaction mixture and mix immediately. 4.Record the increase in A340nm for 2 minutes |
Storage : | Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles. |
Publication : |
Human dihydrofolate reductase influences the sensitivity of the malaria parasite Plasmodium falciparum to ketotifen–A cautionary tale in screening transgenic parasites (2016)
|
Gene Name : | DHFR dihydrofolate reductase [ Homo sapiens ] |
Synonyms : | DHFR; dihydrofolate reductase; EC 1.5.1.3 |
Gene ID : | 1719 |
mRNA Refseq : | NM_000791 |
Protein Refseq : | NP_000782 |
MIM : | 126060 |
UniProt ID : | P00374 |
Chromosome Location : | 5q11.2-q13.2 |
Pathway : | Folate biosynthesis; Metabolic pathways; One carbon pool by folate |
Function : | NADP or NADPH binding; dihydrofolate reductase activity; oxidoreductase activity |
Products Types
◆ Recombinant Protein | ||
DHFR-0618H | Recombinant Human DHFR Protein (M1-D187), His tagged | +Inquiry |
Dhfr-2545M | Recombinant Mouse Dhfr Protein, Myc/DDK-tagged | +Inquiry |
DHFR-2586H | Recombinant Human DHFR Protein, GST-tagged | +Inquiry |
DHFR-2354M | Recombinant Mouse DHFR Protein, His (Fc)-Avi-tagged | +Inquiry |
DHFR-755H | Recombinant Human DHFR Protein, His (Fc)-Avi-tagged | +Inquiry |
◆ Lysates | ||
DHFR-353HCL | Recombinant Human DHFR cell lysate | +Inquiry |
DHFR-6946HCL | Recombinant Human DHFR 293 Cell Lysate | +Inquiry |
◆ Assay kits | ||
Kit-2097 | Dihydrofolate Reductase Colorimetric Activity Kit | +Inquiry |
Kit-2100 | Dihydrofolate Reductase Inhibitor Screening Kit | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Inquiry
0
Inquiry Basket