Recombinant Human DHFR cell lysate

• Cat.No.: DHFR-353HCL

Specification

• Species: Hamster
• Tag: Non
• Tissue Type: Ovary
• Preparation method: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The lysate was adjusted to 2 mg/ml.
• Size: 200 ug
• Recommended Usage: Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
• Storage Buffer: In modified RIPA Lysis Buffer.
• Applications: Western Blot;Immunoprecipitation

Gene Information

• Gene Name: DHFR dihydrofolate reductase [ Homo sapiens ]
• Official Symbol: DHFR
• Synonyms: DYR; DHFRP1
• Gene ID: 1719
• mRNA Refseq: NM_000791
• Protein Refseq: NP_000782
• MIM: 126060
• UniProt ID: P00374
• Chromosome Location: 5q11.2-q13.2
• Pathway: Cell Cycle, organism-specific biosystem; Defective AMN causes hereditary megaloblastic anemia 1, organism-specific biosystem; Defective GIF causes intrinsic factor deficiency, organism-specific biosystem