Recombinant Human N-myc Downstream Regulated 1, His-tagged

PRODUCTS

Home / Products / Recombinant Proteins / Recombinant Human N-myc Downstream Regulated 1, His-tagged

Recombinant Human N-myc Downstream Regulated 1, His-tagged

NDRG1 Related Products

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.

Cat. No. : NDRG1-1398H
Product Overview : Recombinant human NDRG1, fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography. MW =43.9 kDa (402aa).
Description : N-myc downstream regulated gene (NDRG)-1 is one of 4 members of the NDRG α/β-hydrolase family. This protein is classified in databases as a tumor suppressor and heavy metal-response protein. Its functions include cell-cycle regulation, cellular differentiation, apoptosis, hypoxia response and metal-ion sensing. Mutation in the NDRG1 gene has been reported to be causative for hereditary motor and sensory neuropathy-Lom (HMSNL), a severe autosomal recessive form of Charcot-Marie-Tooth (CMT) disease.
Source : E.coli.
Amino Acid Sequence : MPALPEDGGS GAFPPGHFKD PKRLYCKNGG FFLRIHPDGR VDGVREKSDP HIKLQLQAEE RGVVSIKGVC ANRYLAMKED GRLLASKCVT DECFFFERLE SNNYNTYRSR KYTSWYVALK RTGQYKLGSK TGPGQKAILF LPMSAKS
Form : Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 0.1 mM PMSF, 10% Glycerol.
Molecular Weight : 43.9 kDa (402aa), confirmed by MALDI-TOF
Purity : > 95% by SDS – PAGE.
Concentration : 1 mg/ml (determined by Bradford assay).
Biological Activity : The ED50 for this effect is 0.5 - 1.5 ng/ml. Measured in a cell proliferation assay using MCF7 cell.
Activity Assay : 1. Cell line: MCF7 (Human breast adenocarcinoma cell)2. Maintenance Condition: RPMI 1640 containing 10% FBS 3. Assay medium: serum free RPMI 1640 4. Cell density: 2 x 104 cells/well (96 well plate, final volume 100ul) 5. Incubation time : 40 hrs (after sample treatment)6. Concentration range: 0.19ng/ml - 50ng/ml 7. Detection method : BrdU assay
Storage : Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Gene Name : NDRG1 N-myc downstream regulated 1 [ Homo sapiens ]
Synonyms : NDRG1; N-myc downstream regulated 1; GC4; RTP; DRG1; NDR1; NMSL; TDD5; CAP43; CMT4D; HMSNL; RIT42; TARG1; PROXY1; protein regulated by oxygen-1; tunicamycin-responsive protein; differentiation-related gene 1 protein; nickel-specific induction protein Cap43; reducing agents and tunicamycin-responsive protein; DRG-1; DRG1; Protein NDRG1; N-myc downstream-regulated gene 1 protein; Rit42
Gene ID : 10397
mRNA Refseq : NM_001135242
Protein Refseq : NP_001128714
MIM : 605262
UniProt ID : Q92597
Chromosome Location : 8q24
Function : protein binding

Online Inquiry


  •  
  • Note: There will be extra charge for optional service!
  • Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information

Optional Service: Optional requirements on this protein

Other Requirements:

Apply For A Coupon

$50 OFF Your First Purchase

Apply For a Coupon

Enter your email here to subscribe.

creative biomart inc.

Easy access to products and services you need from our library via powerful searching tools.

Follow Us

Copyright © 2007 – 2020 Creative BioMart. All Rights Reserved.