Recombinant Feline Chemokine (C-X-C Motif) Ligand 12(SDF-1b)

• Cat.No.: CXCL12-110F
• Product Overview: Recombinant Feline Stromal Cell-Derived Factor-1 beta produced inE.Coliis a non-glycosylated, Polypeptide chain containing 72 amino acids and having a molecular mass of 8526 Dalton. The SDF-1b is purified by proprietary chromatographic techniques.

Specification

• Cat. No.: CXCL12-110F
• Description: SDF-1 (stromal cell-derived factor-1) is small cytokine belonging to the chemokine family that is officially designated Chemokine (C-X-C motif) ligand 12 (CXCL12). It is produced in two forms, SDF-1α/CXCL12a and SDF-1β/CXCL12b, by alternate splicing of the same gene. Chemokines are characterized by the presence of four conserved cysteines, which form two disulfide bonds. The CXCL12 proteins belong to the group of CXC chemokines, whose initial pair of cysteines are separated by one intervening amino acid. CXCL12 is strongly chemotactic for lymphocytes and has been implicated as an important cell co-ordinator during development. During embryogenesis it directs the migration of hematopoietic cells from foetal liver to bone marrow. Mice which were knocked-out for CXCL12 gene were lethal before the birth or within just 1 hour of life. As another role, CXCL12a alters also the electrophysiology of neurons.
• Source: Escherichia Coli.
• Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Formulation: The protein was lyophilized from a concentrated (1 mg/ml) sterile solution containing no additives.
• Solubility: It is recommended to reconstitute the lyophilized SDF-1b in sterile 18MΩ-cm H₂O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
• Biological Activity: The specific activity as determined by its ability to chemoattract human peripheral T cells stimulated with IL-2 and PHA in a concentration range from 10-75 ng/ml.
• Purity: Greater than 97.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Storage: Lyophilized SDF-1b although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CXCL12 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Gene Information

• Gene Name: CXCL12 chemokine (C-X-C motif) ligand 12 [ Felis catus ]
• Synonyms: CXCL12; chemokine (C-X-C motif) ligand 12; SDF-1; stromal cell-derived factor 1
• Gene ID: 493806
• mRNA Refseq: NM_001009847
• Protein Refseq: NP_001009847
• UniProt ID: O62657

Reviews

06/15/2022

The CXCL12 protein is of exceptional quality, making it an ideal candidate to fulfill my experimental requirements.

01/08/2022

The manufacturer's commitment to providing excellent technical support is truly commendable, as they have proven to be readily available to assist in resolving any concerns or challenges I encountered while working with the protein.

04/26/2021

Its purity and integrity have consistently delivered reliable and robust results in my research.

Q&As

How are researchers using CXCL12 in neurodegenerative disease research?

CXCL12 is being investigated for its role in neuroprotection and neuroregeneration in conditions like Alzheimer's and Parkinson's disease.

Are there diagnostic tests that measure CXCL12 levels in patients?

Yes, ELISA and other assays can be used to measure CXCL12 levels, aiding in the diagnosis and monitoring of certain diseases.

Can CXCL12 be used as a biomarker for specific diseases?

CXCL12 levels can serve as a biomarker for various diseases, such as HIV, cancer, and inflammatory disorders.

How is CXCL12 being researched for cardiovascular therapies?

Scientists are exploring the potential of CXCL12-based therapies to promote vascular repair and treat heart diseases.

Can CXCL12-based therapies help in spinal cord injury treatment?

Researchers are studying CXCL12-based treatments to promote nerve regeneration and functional recovery after spinal cord injuries.