Active Recombinant Human Nerve Growth Factor (Beta Polypeptide)

• Cat.No.: NGF-14H
• Product Overview: Recombinant Human nerve growth factor (beta polypeptide) encoding the human beta-NGF protein sequence (containing the signal peptide, pro-peptide and the mature beta-NGF sequence) was expressed in modifiedhuman 293 cells.

Specification

• Cat. No.: NGF-14H
• Description: Beta-NGF, also known as nerve growth factor beta-1, NGF-b1, or NGF-beta, is a neurotrophic factor that influences the growth and differentiation of sympathetic and sensory neurons. Beta-NGF, comprised alpha, beta, and gamma subunits, is a 26 kDa non-covalently associated homodimer with 2 potential N-linked glycosylation sites.
• Source: Human 293 cells.
• Amino Acid Sequence: YAEHKSHRGEYSVCDSESLWVTDKS SAIDIRGHQVTVLGEIKTGNSPVKQ YFYETRCKEAR PVKNGCRGIDDKHWNSQCKTSQTYV RALTSENNKLVGWRWIRIDTSCVCA LSRKIGRT.
• Molecular Mass: Beta-NGFmigrates at approximately 12-16 kDa in SDS-PAGE. This compares with the predicted molecular mass of 13.5 kDa.
• pI: Beta-NGF separates into a number of isoforms with a pI between 9 and 10 in 2D PAGE due to post-translational modifications. This compares with the unmodified beta-NGF that has a predicted pI of 9.
• Purity: >95%, as determined by SDS-PAGE and visualized by silver stain.
• Formulation: When reconstituted in 0.5 ml sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose.
• Reconstitution: It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial.
• Storage: Lyophilized products should be stored at 2 to 8°C. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
• Activity: The ED50 of beta-NGF is typically 0.2-1.0 ng/ml as measured in a cell proliferation assay using the human growth factor dependent TF-1 cell line.

Gene Information

• Gene Name: NGF nerve growth factor (beta polypeptide) [ Homo sapiens ]
• Synonyms: NGF; nerve growth factor (beta polypeptide); NGFB; HSAN5; Beta-NGF; MGC161426; MGC161428; Beta-nerve growth factor; nerve growth factor, beta subunit; nerve growth factor, beta polypeptide; beta-nerve growth factor; OTTHUMP00000013653
• Gene ID: 4803
• mRNA Refseq: NM_002506
• Protein Refseq: NP_002497
• UniProt ID: P01138
• Chromosome Location: 1p13.1
• MIM: 162030
• Pathway: Apoptosis; MAPK signaling pathway; Neurotrophin signaling pathway; Signalling by NGF
• Function: growth factor activity; rve growth factor receptor binding; receptor signaling protein activity