Recombinant Human PRKACA, GST-tagged, Active

• Cat.No.: PRKACA-394H
• Product Overview: Recombinant human full-length PKAcalpha was expressed by baculovirus inSf9 cellsusing an N-terminal GST tag. MW=69 kDa.

Specification

• Cat. No.: PRKACA-394H
• Description: The catalytic subunit C-alpha of PKA (PKAcα) is a member of the Ser/Thr protein kinase family and has been assigned to chromosome region 19p13.1. Null mutation in PKAcα leads to early postnatal lethality in the majority of C-alpha knockout mice. Surprisingly, a small percentage of C-alpha knockout mice, although runted, survived to adulthood. In these animals, compensatory increases in C-beta levels occurred in brain whereas many tissues, including skeletal muscle, heart, and sperm, contained less than 10% of the normal PKA activity.
• Source: Sf9 insect cells using baculovirus.
• Sequence: Full-length.
• Applications: Kinase Assay, Western Blot.
• Storage And Stability: Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Gene Information

• Gene Name: PRKACA protein kinase, cAMP-dependent, catalytic, alpha [ Homo sapiens ]
• Synonyms: PRKACA; protein kinase, cAMP-dependent, catalytic, alpha; PKACA; MGC48865; MGC102831; PKACa; cAMP-dependent protein kinase catalytic subunit alpha; EC 2.7.11.11; PKA C-alpha
• Gene ID: 5566
• mRNA Refseq: NM_002730
• Protein Refseq: NP_002721
• UniProt ID: P17612
• Chromosome Location: 19p13.1
• MIM: 601639
• Pathway: Apoptosis; Calcium signaling pathway; Chemokine signaling pathway; Dilated cardiomyopathy; Gap junction; GnRH signaling pathway; Hedgehog signaling pathway; Insulin signaling pathway; Melanogenesis; Long-term potentiation; Progesterone-mediated oocyte maturation; Wnt signaling pathway; Vibrio cholerae infection
• Function: ATP binding; nucleotide binding; transferase activity; cAMP-dependent protein kinase activity; cAMP-dependent protein kinase inhibitor activity; protein serine/threonine kinase activity; protein kinase binding