||Recombinant human full-length PKAcalpha was expressed by baculovirus inSf9 cellsusing an N-terminal GST tag. MW=69 kDa.
||The catalytic subunit C-alpha of PKA (PKAcα) is a member of the Ser/Thr protein kinase family and has been assigned to chromosome region 19p13.1. Null mutation in PKAcα leads to early postnatal lethality in the majority of C-alpha knockout mice. Surprisingly, a small percentage of C-alpha knockout mice, although runted, survived to adulthood. In these animals, compensatory increases in C-beta levels occurred in brain whereas many tissues, including skeletal muscle, heart, and sperm, contained less than 10% of the normal PKA activity.
||Sf9 insect cells using baculovirus.
||Kinase Assay, Western Blot.
|Storage And Stability:
||Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.