||Recombinant human full-length PKCbeta I was expressed by baculovirus inSf9 cellsusing an N-terminal GST tag. MW=102 kDa.
||PKCβI is a member of the PKC family (phospholipid-dependent serine/threonine kinase) and is highly related to PKCβ II. Unlike the mature PKCβ II mRNA and protein, which rapidly increase following acute insulin treatment, the PKCβI mRNA and protein levels remain unchanged. The stable overexpression of PKCbetaII, but not PKCβI, leads to insulin-stimulated glucose uptake into cells. Upon stimulation of B lymphocytes and mast cells, Syk regulates Btk, and Btk selectively regulates enzymatic activity of PKCβI. Specific regulation of PKCβI by Btk is consistent with the selective association of Btk with PKCβI.
||Sf9 insect cells using baculovirus.
||Kinase Assay, Western Blot.
|Storage And Stability:
||Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.