Recombinant Human PDZ Binding Kinase, GST-tagged, Active

• Cat.No.: PBK-447H
• Product Overview: Recombinant full-length human TOPK was expressed by baculovirus inSf9insect cells using an N-terminal GST tag. MW = 68 kDa.

Specification

• Cat. No.: PBK-447H
• Description: TOPK is a MAPK kinase that phosphorylates p38 MAPK and is activated in a cell-cycle-dependent manner in neuronal progenitor cells in vitro. Expression of TOPK is detected in male germ line progenitor cells, activated T-cells, and a variety of lymphomas and leukemias. In vitro studies have shown that activated TOPK phosphorylated p38MAPK but not JNK or ERK. TOPK activation requires phosphorylation by both the M-phase CDK1/CyclinB kinase complex and another unknown kinase, possibly RafC or RafA. TOPK may play an important role in linking extracellular signals to an intracellular state, possibly allowing extracellular influence on the cell-cycle-related processes of proliferation or differentiation.
• Source: Sf9 insect cells using baculovirus.
• Sequence: Full-length.
• Applications: Kinase Assay, Western Blot.
• Storage And Stability: Store product at -70℃. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Gene Information

• Gene Name: PBK PDZ binding kinase [ Homo sapiens ]
• Synonyms: PBK; PDZ binding kinase; SPK; CT84; TOPK; Nori-3; FLJ14385; PDZ-binding kinase; cancer/testis antigen 84; MAPKK-like protein kinase; serine/threonine protein kinase; T-LAK cell-originated protein kinase; spermatogenesis-related protein kinase; EC 2.7.12.2; Lymphokine-activated killer T-cell-originated protein kinase
• Gene ID: 55872
• mRNA Refseq: NM_018492
• Protein Refseq: NP_060962
• MIM: 611210
• UniProt ID: Q96KB5
• Chromosome Location: 8p21.2
• Function: ATP binding; nucleotide binding; protein binding; protein serine/threonine kinase activity; transferase activity