Recombinant Mouse Vascular Endothelial Growth Factor A 164AA

• Cat.No.: Vegfa-464M
• Product Overview: Recombinant mouse Vascular endothelial growth factor is a disulfide-linked homodimer protein consisting of 165 amino acid residue subunits, and migrates as an approximately 28 kDa protein under non-reducing and as 14kDa under reducing conditions in SDS-PAGE. It was expressed inE.coli.

Specification

• Cat. No.: Vegfa-464M
• Description: VEGF is a homodimeric heavily glycosylated protein.The human factor occurs in several molecular variants of 121, 162, 145, 148, 165, 183, 189 , 206 amino acids, arising by alternative splicing of the mRNA.The splice forms of VEGF differ in biological properties such as the receptor types, which they recognize and their interaction with heparan sulfate proteoglycans. The 165 amino acid form of the factor is the most common form in most tissues. The interaction of VEGF with heparin-like molecules of the extracellular matrix is required for efficient receptor binding. Protamine sulfate and suramin are capable of replacing the receptor-bound factor. The high-affinity receptor for VEGF, now known as VEGFR1, has been identified as the gene product of the FLT-1. Another receptor for VEGF, now known as VEGFR2, is KDR, also known as FLK-1. A factor that competes with the 165 amino acid form of VEGF for receptor binding is PLGF. A third receptor type, VEGFR3 is known also as FLT- 4. An isoform-specific receptor for VEGF165 has been identified as human Neuropilin-1.
• Source: Escherichia Coli.
• Purity: >95%, as determined by SDS-PAGE and HPLC.
• Biological Activity: The ED(50) was determined by the dose-dependent proliferation of human umbilical vein endothelial cells and was found to be in the range of 2.0-4.0 ng/ml.
• Protein Sequence: MNFLLSWVHW TLALLLYLHH AKWSQAAPTT EGEQKSHEVI KFMDVYQRSY CR PIE TLVDI FQEYPDEIEY IFKPSCVPLM RCAGCCNDEA LECVPTSESN ITMQ IMRIKP HQSQHIGEMS FLQHSRCECR PKKDRTKPEN HCEPCSERRK HL FV QDPQTC KCSCKNTDSR CKARQLELNE RTCRCDKPRR.
• Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
• Presentation: Recombinant mouse VEGF was lyophilized from 0.2 μm filtered PBS solution, pH 7.0.
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Storage: The lyophilized protein is stable for at least 2 years from date of receipt at -20°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2°C - 8°C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Gene Information

• Gene Name: Vegfa vascular endothelial growth factor A [ Mus musculus ]
• Synonyms: Vegfa; vascular endothelial growth factor A; Vpf; Vegf; Vegf-a; Vegf120; Vegf164; VEGF120; VPF; Vegf188; OTTMUSP00000017463; VEGF164; OTTMUSP00000017464; VEGF-A; OTTMUSP00000022243; vascular permeability factor; VEGF188; MGC70609; MVCD1; VEGF; Vascular permeability factor; vascular endothelial growth factor; vascular endothelial growth factor isoform VEGF165
• Gene ID: 22339
• mRNA Refseq: NM_001025250
• Protein Refseq: NP_001020421
• UniProt ID: Q00731
• Chromosome Location: 17 C; 17 24.2 cM
• Pathway: Bladder cancer; Focal adhesion; Cytokine-cytokine receptor interaction; Pancreatic cancer; Pathways in cancer; Renal cell carcinoma; VEGF signaling pathway; mTOR signaling pathway
• Function: growth factor activity; heparin binding

Reviews

04/21/2021

VEGFA's catalytic activity is so strong that it can adapt even in the face of complex chemical reactions.

03/09/2020

VEGFA they produced was highly active and the experimental effect was obvious.

Q&As

How does VEGFA protein relate to other biomarkers?

Studies have shown that there is an association between the expression level of VEGFA protein and other biomarkers, for example, it can be associated with indicators such as tumor cell differentiation, metastatic capacity, and prognosis.

How to harness the activity of VEGFA protein for disease treatment?

At present, a variety of drugs have been developed to target the VEGFA protein and its related signaling pathway, including inhibiting its expression and inhibiting its binding to receptors, etc., for the treatment of tumors and other vascular-related diseases.

What is the role of VEGFA protein in tumor angiogenesis?

VEGFA protein plays a key role in tumor angiogenesis, promoting tumor growth and metastasis by stimulating endothelial cell proliferation, migration, and vascular network formation.

How well is the VEGFA protein expressed in different tissues and organs?

The expression of VEGFA protein in different tissues and organs is different, and it is expressed in tumor tissue, cardiovascular system and other tissues and organs, and the expression level may be affected by many factors.

What is the relationship between the expression level of VEGFA protein and the degree of tumor malignancy?

Studies have shown that the expression level of VEGFA protein is related to the degree of malignancy of the tumor, and the high expression of VEGFA protein often indicates that the tumor is more aggressive.

How about the fuction of VEGFA protein in cardiovascular disease?

VEGFA protein plays an important role in cardiovascular disease by regulating endothelial cell function, promoting vascular repair and maintaining cardiovascular system homeostasis.