||Recombinant murine CTACK produced inE.coliis a 10.9 kDa protein containing 95 amino acid residues.
||CTACK is a keratinocyte-derived CC chemokine which signals through the CCR10 receptor. Both CTACK and CCR10 are expressed in normal and irritated epithelial cells. CTACK selectively attracts CLA+ T-cells and directs them into the skin. CTACK contains the four highly conserved cysteine residues present in most CC chemokines. The mature protein contains 88 amino acid residues.
||Greater than 98% (determined by SDS-PAGE and HPLC analysis).
||< 0.1 ng per μg (1EU/μg) of rMu CTACK.
||Determined by its ability to chemoattract CXCR3 transfected HEK/293 cells using a concentration range of 10.0-100.0 ng/ml.
||Lyophilized from a sterile-filtered solution with no additives.
||Please Note: Always centrifuge product briefly before opening the vial. Reconstitute in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can be diluted into other aqueous buffers and stored at -20°C for future use.
|Storage & Stability:
||The lyophilized powder, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted CTACK should be used immediately or stored in undiluted working aliquots at -20°C. Avoid repeated freeze / thaw cycles.