Active Recombinant Human Transforming Growth Factor, Beta 3, Dimeric Form

• Cat.No.: TGFB3-468H
• Product Overview: Recombinant human TGFβ-3 is a 25.4 kDa protein composed of two identical 112 amino acid peptide chains linked by a single disulfide bond. It was expressed inNicotiana benthamiana.Recombinant human TGFβ-3 is purified by sequential chomatograpy (FPLC). This product contains no animal –derived components or impurities.

Specification

• Cat. No.: TGFB3-468H
• Description: Transforming growth factor –β is a family of five related cytokines that have been shown on a wide variety of normal and neoplastic cells, indicating the importance of these homodimeric proteins as multi-functional regulators of cellular activity. The three mammalian isoforms of TGFβ (TGFβ-1, TGFβ-2 and TGFβ-3 ) signal through the same receptor and elicit similar biological responses. They are involved in physiological processes as embryogenesis, tissue remodelling and wound healing.
• Source: Nicotiana benthamiana.
• Molecular Formula: C 600H902N166O174S10.
• Extinction Coefficient: E0.1% = 1.729 (A280 nm).
• P.I: 6.75.
• Purity Assay: >95 % as determinate by SDS-PAGE gel.
• Endotoxin Level: < 0.04 EU/ µg protein (LAL method).
• Formulation: Lyophilized from a buffer of Tris HCl 0.05M pH 7.4.
• Activity: The biological activity of TGF-β3 is measured in culture by its ability to inhibit the mink lung epithelial cells proliferation. (ED50 is defined as the effective concentration of growth factor that elicits a 50% inhibition in cell growth in a cell based bioassay).
• Stotage And Stability: This lyophilized preparation is stable at 2-8° C, but should be kept at –20°C for long term storage. Repeated freezing and thawing is not recommended.

Gene Information

• Gene Name: TGFB3 transforming growth factor, beta 3 [ Homo sapiens ]
• Synonyms: Transforming Growth Factor-beta3; TGFB3; ARVD; FLJ16571; TGF-beta3; transforming growth factor, beta 3
• Gene ID: 7043
• mRNA Refseq: NM_003239
• Protein Refseq: NP_003230
• MIM: 190230
• UniProt ID: P10600
• Chromosome Location: 4q24
• Pathway: Cell cycle;Chronic myeloid leukemia; Colorectal cancer; Cytokine-cytokine receptor interaction; Hypertrophic cardiomyopathy (HCM); MAPK signaling pathway; Pancreatic cancer;Pathways in cancer; Renal cell carcinoma; TGF-beta signaling pathway
• Function: Growth factor activity; Identical protein binding; Protein heterodimerization activity; Transforming growth factor beta binding; Type I transforming growth factor beta receptor binding; Type II transforming growth factor beta receptor binding