Recombinant Human ALKBH3, His-tagged

• Cat.No.: ALKBH3-26501TH
• Product Overview: Recombinant full length Human ALKBH3 with N terminal His and DDDDK tags; 325 amino acids with tags, Predicted MWt 37.9 kDa.

Specification

• Description: The Escherichia coli AlkB protein protects against the cytotoxicity of methylating agents by repair of the specific DNA lesions generated in single-stranded DNA. ALKBH2 (MIM 610602) and ALKBH3 are E. coli AlkB homologs that catalyze the removal of 1-methyladenine and 3-methylcytosine (Duncan et al.
• Protein length: 286 amino acids
• Conjugation: HIS
• Molecular Weight: 37.900kDa inclusive of tags
• Source: E. coli
• Tissue specificity: Ubiquitous. Detected in heart, pancreas, skeletal muscle, thymus, testis, ovary, spleen, prostate, small intestine, peripheral blood leukocytes, urinary bladder and colon.
• Form: Liquid
• Purity: >95% by SDS-PAGE
• Storage buffer: Preservative: NoneConstituents: 10% Glycerol, 0.1M Sodium chloride, 20mM Tris HCl, 2mM DTT, pH 8.0
• Storage: Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Sequences of amino acids: MRGSHHHHHHGMASMTGGQQMGRDL YDDDDKDRWGSELDMEEKRRRARVQ GAWAAPVKSQAIAQPATTAKSHLHQ KPGQTWKNKEHHLSDREFVFKEPQQ VVRRAPEPRVIDREGVYEISLSPTG VSRVCLYPGFVDVKEADWILEQLCQ DVPWKQRTGIREDITYQQPRLTAWY GELPYTYSRITMEPNPHWHPVLRTL KNRIEENTGHTFNSLLCNLYRNEKD SVDWHSDDEPSLGRCPIIASLSFGA TRTFEMRKKPPPEENGDYTYVERVK IPLDHGTLLIMEGATQADWQHRVPK EYHSREPRVNLTFRTVYPDPRGAPW
• Sequence Similarities: Belongs to the alkB family.Contains 1 Fe2OG dioxygenase domain.

Gene Information

• Gene Name: ALKBH3 alkB, alkylation repair homolog 3 (E. coli) [ Homo sapiens ]
• Official Symbol: ALKBH3
• Synonyms: ALKBH3; alkB, alkylation repair homolog 3 (E. coli); alpha-ketoglutarate-dependent dioxygenase alkB homolog 3; DEPC 1
• Gene ID: 221120
• mRNA Refseq: NM_139178
• Protein Refseq: NP_631917
• MIM: 610603
• Uniprot ID: Q96Q83
• Chromosome Location: 11p11.2
• Pathway: ABH3 mediated Reversal of Alkylation Damage, organism-specific biosystem; DNA Damage Reversal, organism-specific biosystem; DNA Repair, organism-specific biosystem; Reversal of Alkylation Damage By DNA Dioxygenases, organism-specific biosystem
• Function: DNA-N1-methyladenine dioxygenase activity; L-ascorbic acid binding; cytosine C-5 DNA demethylase activity; ferrous iron binding; metal ion binding

Reviews

04/30/2021

Structure analysis by nuclear magnetic resonance (NMR), X-ray crystallography or electron microscopy showed that ALKBH3 was stable.

04/28/2021

Prone to induce immune responses.

05/11/2019

Stable under special conditions.

Q&As

What diseases is the mutation of ALKBH3 gene associated with?

ALKBH3 gene mutation is associated with colon and rectal cancer.

What factors are related to the stability of ALKBH3 recombinant protein?

The stability of ALKBH3 recombinant protein is related to freezing temperature, pH value and ionic strength.

What are the biological functions of human ALKBH3 recombinant protein?

ALKBH3 is a DNA repair enzyme that repairs methyl groups along DNA strands.

Is ALKBH3 mutation associated with recurrent liver cancer?

No studies have shown that ALKBH3 mutations are associated with recurrent liver cancer.

Is ALKBH3 gene involved in drug metabolism?

At present, there is no clear study that ALKBH3 gene is related to drug metabolism.

Can ALKBH3 gene be used as a molecular marker for the prognosis of liver cancer?

ALKBH3 gene can be used as one of the molecular markers of liver cancer prognosis.

Does ALKBH3 have immunomodulatory effects?

There is no clear evidence that ALKBH3 recombinant protein has immunomodulatory effects.

Are polymorphisms of ALKBH3 gene associated with any diseases?

ALKBH3 gene polymorphism is associated with various cancers such as lung cancer, esophageal cancer and colon cancer.

Does ALKBH3 mutation affect DNA damage repair function?

Mutations in ALKBH3 may affect the function of DNA damage repair.

What is the stability of ALKBH3 recombinant protein?

ALKBH3 recombinant protein should be stored in a -80℃ refrigerator to ensure stability.

What is the role of ALKBH3 recombinant protein in cancer treatment?

ALKBH3 recombinant protein can help predict the therapeutic effect and prognosis of some cancers.

Does ALKBH3 gene affect human metabolism?

ALKBH3 has not been found to affect human metabolic capacity.

Does ALKBH3 have an effect on the immune system?

No studies have shown that ALKBH3 has an important effect on the immune system.

Does the recommended dosage of ALKBH3 recombinant protein?

There are no clear dosage guidelines at present, and further research is needed to determine.

Is ALKBH3 related to treatment response of liver cancer?

ALKBH3 mutation may affect the treatment response of liver cancer.

Can ALKBH3 gene variation be used as an early warning indicator of cancer?

There is no strong evidence that the ALKBH3 gene variant can be used as an early warning indicator of cancer.

Can ALKBH3 recombinant protein be used as a target for cancer treatment?

The recombinant ALKBH3 protein may be a potential target for the treatment of some cancers, but further research is needed to confirm it.