Recombinant Human ALKBH3, His-tagged

• Cat.No.: ALKBH3-26501TH
• Product Overview: Recombinant full length Human ALKBH3 with N terminal His and DDDDK tags; 325 amino acids with tags, Predicted MWt 37.9 kDa.

Specification

• Description: The Escherichia coli AlkB protein protects against the cytotoxicity of methylating agents by repair of the specific DNA lesions generated in single-stranded DNA. ALKBH2 (MIM 610602) and ALKBH3 are E. coli AlkB homologs that catalyze the removal of 1-methyladenine and 3-methylcytosine (Duncan et al.
• Protein length: 286 amino acids
• Conjugation: HIS
• Molecular Weight: 37.900kDa inclusive of tags
• Source: E. coli
• Tissue specificity: Ubiquitous. Detected in heart, pancreas, skeletal muscle, thymus, testis, ovary, spleen, prostate, small intestine, peripheral blood leukocytes, urinary bladder and colon.
• Form: Liquid
• Purity: >95% by SDS-PAGE
• Storage buffer: Preservative: NoneConstituents: 10% Glycerol, 0.1M Sodium chloride, 20mM Tris HCl, 2mM DTT, pH 8.0
• Storage: Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Sequences of amino acids: MRGSHHHHHHGMASMTGGQQMGRDL YDDDDKDRWGSELDMEEKRRRARVQ GAWAAPVKSQAIAQPATTAKSHLHQ KPGQTWKNKEHHLSDREFVFKEPQQ VVRRAPEPRVIDREGVYEISLSPTG VSRVCLYPGFVDVKEADWILEQLCQ DVPWKQRTGIREDITYQQPRLTAWY GELPYTYSRITMEPNPHWHPVLRTL KNRIEENTGHTFNSLLCNLYRNEKD SVDWHSDDEPSLGRCPIIASLSFGA TRTFEMRKKPPPEENGDYTYVERVK IPLDHGTLLIMEGATQADWQHRVPK EYHSREPRVNLTFRTVYPDPRGAPW
• Sequence Similarities: Belongs to the alkB family.Contains 1 Fe2OG dioxygenase domain.

Gene Information

• Gene Name: ALKBH3 alkB, alkylation repair homolog 3 (E. coli) [ Homo sapiens ]
• Official Symbol: ALKBH3
• Synonyms: ALKBH3; alkB, alkylation repair homolog 3 (E. coli); alpha-ketoglutarate-dependent dioxygenase alkB homolog 3; DEPC 1
• Gene ID: 221120
• mRNA Refseq: NM_139178
• Protein Refseq: NP_631917
• MIM: 610603
• Uniprot ID: Q96Q83
• Chromosome Location: 11p11.2
• Pathway: ABH3 mediated Reversal of Alkylation Damage, organism-specific biosystem; DNA Damage Reversal, organism-specific biosystem; DNA Repair, organism-specific biosystem; Reversal of Alkylation Damage By DNA Dioxygenases, organism-specific biosystem
• Function: DNA-N1-methyladenine dioxygenase activity; L-ascorbic acid binding; cytosine C-5 DNA demethylase activity; ferrous iron binding; metal ion binding