"NT5C2" Related Products

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Recombinant Human 5 -Nucleotidase, Cytosolic II, T7-tagged

Cat. No.: NT5C2-395H
Product Overview: Recombinant Human NT5C2 was expressed inE.Coliusing an N-terminal T7 tag. MW=64.5 kDa.
Description: Purine 5-prime-nucleotidase preferentially hydrolyzes inosine 5-prime-monophosphate (IMP) and other purine nucleotides, and is allosterically activated by various compounds, including ATP. The enzyme is exclusively located in the cytoplasmic matrix of cells and may have a critical role in the maintenance of a constant composition of intracellular purine/pyrimidine nucleotides in cooperation with other nucleotidases.
Source: E.Coli.
Sequence: 1-561.
Purity: ~95%.
Specific Activity: n/aH.
Applications: This recombinant protein can be used for WB, ELISA, MS and neutralization assays.
Buffer: 10 mM Tris, pH 8.0, 0.1% Triton X-100, 0.002% NaN3, 10mM DTT.
Storage: Store at -70?C. As with any protein, exposing NT5C2 recombinant protein to repeated freeze/thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
SDS-PAGE: SDS PAGE Analysis of NT5C2 Recombinant Protein. 4-20% SDS gradient gel. Coomassie blue staining.
Gene Name: NT5C2 5"-nucleotidase, cytosolic II [ Homo sapiens ]
Synonyms: NT5C2; 5"-nucleotidase, cytosolic II; GMP; NT5B; PNT5; cN-II; IMP-specific 5"-NT; purine 5" nucleotidase; 5"-nucleotidase (purine), cytosolic type B; EC 3.1.3.5
Gene ID: 22978
mRNA Refseq: NM_001134373
Protein Refseq: NP_001127845
MIM: 600417
UniProt ID: P49902
Chromosome Location: 10q24.32-q24.33
Pathway: Metabolic pathways; Nicotinate and nicotinamide metabolism; Purine metabolism; Pyrimidine metabolism; Metablism of nucleotides
Function: 5"-nucleotidase activity; hydrolase activity; magnesium ion binding; nucleotide binding; protein binding

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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