Recombinant Human ARAF

• Cat.No.: ARAF-26034TH
• Product Overview: Recombinant fragment, corresponding to amino acids 282-606 of Human A RAF, containing the mutations YY301-302DD, with an N-terminal proprietary tag, expressed in a Baculovirus infected Sf9 cell expression system, 63.6 kDa.

Specification

• Description: This proto-oncogene belongs to the RAF subfamily of the Ser/Thr protein kinase family, and maybe involved in cell growth and development.
• Tissue specificity: Predominantly in urogenital tissues.
• Biological activity: Specific Activity: 50 nM A RAF yields 8% conversion of 1 uM ATP using MEK1 (K97R) as substrate.
• Form: Liquid
• Storage buffer: Preservative: NoneConstituents: 50% Glycerol, 0.05% Tween 20, 3mM DTT, 25mM Tris HCl, 100mM Sodium chloride, pH 8.0
• Storage: Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
• Sequence Similarities: Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.Contains 1 phorbol-ester/DAG-type zinc finger.Contains 1 protein kinase domain.Contains 1 RBD (Ras-binding) domain.

Gene Information

• Gene Name: ARAF v-raf murine sarcoma 3611 viral oncogene homolog [ Homo sapiens ]
• Official Symbol: ARAF
• Synonyms: ARAF; v-raf murine sarcoma 3611 viral oncogene homolog; ARAF1, v raf murine sarcoma 3611 viral oncogene homolog 1; serine/threonine-protein kinase A-Raf
• Gene ID: 369
• mRNA Refseq: NM_001654
• Protein Refseq: NP_001645
• MIM: 311010
• Uniprot ID: P10398
• Chromosome Location: Xp11.3-p11.23
• Pathway: Acute myeloid leukemia, organism-specific biosystem; Acute myeloid leukemia, conserved biosystem; Bladder cancer, organism-specific biosystem; Bladder cancer, conserved biosystem; Chronic myeloid leukemia, organism-specific biosystem
• Function: ATP binding; metal ion binding; nucleotide binding; protein binding; protein kinase activity