Recombinant Human C4B

• Cat.No.: C4B-27789TH
• Product Overview: Recombinant fragment corresponding to amino acids 1347-1446 of Human C4b with a proprietary tag; predicted MWt 36.63kDa inclusive of tag.

Specification

• Description: This gene encodes the basic form of complement factor 4, part of the classical activation pathway. The protein is expressed as a single chain precursor which is proteolytically cleaved into a trimer of alpha, beta, and gamma chains prior to secretion. The trimer provides a surface for interaction between the antigen-antibody complex and other complement components. The alpha chain may be cleaved to release C4 anaphylatoxin, a mediator of local inflammation. Deficiency of this protein is associated with systemic lupus erythematosus. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. Varying haplotypes of this gene cluster exist, such that individuals may have 1, 2, or 3 copies of this gene. In addition, this gene exists as a long form and a short form due to the presence or absence of a 6.4 kb endogenous HERV-K retrovirus in intron 9.
• Protein length: 100 amino acids
• Molecular Weight: 36.630kDa inclusive of tags
• Source: Wheat germ
• Form: Liquid
• Purity: Proprietary Purification
• Storage buffer: pH: 8.00Constituents:0.31% Glutathione, 0.79% Tris HCl
• Storage: Shipped on dry ice. Upon delivery aliquot and store at -80oC. Avoid freeze / thaw cycles.
• Sequences of amino acids: NNRQIRGLEEELQFSLGSKINVKVG GNSKGTLKVLRTYNVLDMKNTTCQD LQIEVTVKGHVEYTMEANEDYEDYE YDELPAKDDPDAPLQPVTPLQLFEG
• Sequence Similarities: Contains 1 anaphylatoxin-like domain.Contains 1 NTR domain.

Gene Information

• Gene Name: C4B complement component 4B (Chido blood group) [ Homo sapiens ]
• Official Symbol: C4B
• Synonyms: C4B; complement component 4B (Chido blood group); complement component 4B; complement C4-B; C4B1; C4B3; C4F; CH; CO4; CPAMD3
• Gene ID: 721
• mRNA Refseq: NM_001002029
• Protein Refseq: NP_001002029
• MIM: 120820
• Uniprot ID: P0C0L5
• Chromosome Location: 6p21.3
• Pathway: Complement Activation, Classical Pathway, organism-specific biosystem; Complement and coagulation cascades, organism-specific biosystem; Complement and coagulation cascades, conserved biosystem; Pertussis, organism-specific biosystem; Pertussis, conserved biosystem
• Function: endopeptidase inhibitor activity

Reviews

09/05/2021

C4B labeling works very well and can be fluorescently labeled, etc., which is very suitable for our research.

02/04/2021

In experiments, biocompatibility showed up very well.

10/28/2020

Very good at western-blot experimenting.

Q&As

What are the clinical applications of C4B inhibitors?

C4B inhibitors can be used to treat diseases associated with complement hyperactivation, such as autoimmune diseases and graft-versus-host disease. By inhibiting the activity of C4B, it is possible to attenuate the excessive inflammatory response and tissue damage.

How is the expression level of C4B regulated?

The expression level of C4B is regulated by a variety of factors, including inflammatory factors, cytokines, and drugs. These factors can affect the synthesis and release of C4B, thus affecting its function in the immune system.

Is C4B involved in the formation and clearance of immune complexes?

It can bind to other complement components and immunoglobulins to form immune complexes. These immune complexes can be recognized and cleared by clearance mechanisms in the body, such as phagocytes.

Is there a relationship between C4B and other immune system components?

It is closely related to other immune system components (such as T cells, B cells, antibodies, etc.) and is involved in the immune defense response in the body. The interactions and influences between them are important for maintaining immune homeostasis.

How does C4B function differently in different cell types?

The role of C4B in different types of cells may vary. For example, in immune cells, it can be involved in the regulation of immune responses and cell activation, while in endothelial cells, it may be involved in the process of vascular inflammation and injury.

How does C4B interact with other complement components?

C4B interacts with other complement components (e.g., C4A, C2, C3, etc.) to form part of the complement activation pathway and is involved in the immune defense response.