Recombinant Human CBS, His-tagged

• Cat.No.: CBS-27861TH
• Product Overview: Recombinant fragment, corresponding to amino acids 242-551 of Human CBS with N terminal His tag; MWt 38kDa.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 242-551 a.a.
• Description: The protein encoded by this gene acts as a homotetramer to catalyze the conversion of homocysteine to cystathionine, the first step in the transsulfuration pathway. The encoded protein is allosterically activated by adenosyl-methionine and uses pyridoxal phosphate as a cofactor. Defects in this gene can cause cystathionine beta-synthase deficiency (CBSD), which can lead to homocystinuria. Multiple alternatively spliced transcript variants have been found for this gene.
• Conjugation: HIS
• Tissue specificity: In the adult strongly expressed in liver and pancreas, some expression in heart and brain, weak expression in lung and kidney. In the fetus, expressed in brain, liver and kidney.
• Form: Lyophilised:Reconstitute with 153 μl aqua dest.
• Storage buffer: Preservative: NoneConstituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -80oC. Avoid freeze / thaw cycles.
• Sequences of amino acids: QQCDGKLDMLVASVGTGGTITGIARKLKEKCPGCRIIGVD PEGSILAEPEELNQTEQTTYEVEGIGYDFIPTVLDRTV VDKWFKSNDEEAFTFARMLIAQEGLLCGGSAGSTVAVA VKAAQELQEGQRCVVILPDSVRNYMTKFLSDRWMLQKG FLKEEDLTEKKPWWWHLRVQELGLSAPLTVLPTITCGHTI EILREKGFDQAPVVDEAGVILGMVTLGNMLSSLLAGKV QPSDQVGKVIYKQFKQIRLTDTLGRLSHILEMDHFALV VHEQIQYHSTGKSSQRQMVFGVVTAIDLLNFVAAQERD QK
• Sequence Similarities: Belongs to the cysteine synthase/cystathionine beta-synthase family.Contains 1 CBS domain.

Gene Information

• Gene Name: CBS cystathionine-beta-synthase [ Homo sapiens ]
• Official Symbol: CBS
• Synonyms: CBS; cystathionine-beta-synthase; cystathionine beta-synthase; HIP4
• Gene ID: 875
• mRNA Refseq: NM_000071
• Protein Refseq: NP_000062
• MIM: 613381
• Uniprot ID: P35520
• Chromosome Location: 21q22.3
• Pathway: Cysteine and methionine metabolism, organism-specific biosystem; Cysteine and methionine metabolism, conserved biosystem; Cysteine biosynthesis, homocysteine + serine => cysteine, organism-specific biosystem; Cysteine biosynthesis, homocysteine + serine =>
• Function: cystathionine beta-synthase activity; enzyme binding; heme binding; heme binding; lyase activity