Recombinant Full Length Human CBS Protein, C-Flag-tagged

• Cat.No.: CBS-1191HFL
• Product Overview: Recombinant Full Length Human CBS Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene acts as a homotetramer to catalyze the conversion of homocysteine to cystathionine, the first step in the transsulfuration pathway. The encoded protein is allosterically activated by adenosyl-methionine and uses pyridoxal phosphate as a cofactor. Defects in this gene can cause cystathionine beta-synthase deficiency (CBSD), which can lead to homocystinuria. This gene is a major contributor to cellular hydrogen sulfide production. Multiple alternatively spliced transcript variants have been found for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 60.4 kDa
• AA Sequence: MPSETPQAEVGPTGCPHRSGPHSAK GSLEKGSPEDKEAKEPLWIRPDAPS RCTWQLGRPASESPHHHTPP AKSPKILPDILKKIGDTPMVRINKI GKKFGLKCELLAKCEFFNAGGSVKD RISLRMIEDAERDGTLKPGD TIIEPTSGNTGIGLALAAAVRGYRC IIVMPEKMSSEKVDVLRALGAEIVR TPTNARFDSPESHVGVAWRL KNEIPNSHILDQYRNASNPLAHYDT TADEILQQCDGKLDMLVASVGTGGT ITGIARKLKEKCPGCRIIGV DPEGSILAEPEELNQTEQTTYEVEG IGYDFIPTVLDRTVVDKWFKSNDEE AFTFARMLIAQEGLLCGGSA GSTVAVAVKAAQELQEGQRCVVILP DSVRNYMTKFLSDRWMLQKGFLKEE DLTEKKPWWWHLRVQELGLS APLTVLPTITCGHTIEILREKGFDQ APVVDEAGVILGMVTLGNMLSSLLA GKVQPSDQVGKVIYKQFKQI
  RLTDTLGRLSHILEMDHFALVVHEQ IQYHSTGKSSQRQMVFGVVTAIDLL NFVAAQERDQKTRTRPLEQKLISEE DLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome
• Protein Pathways: Cysteine and methionine metabolism, Glycine, serine and threonine metabolism, Metabolic pathways, Selenoamino acid metabolism

Gene Information

• Gene Name: CBS cystathionine beta-synthase [ Homo sapiens (human) ]
• Official Symbol: CBS
• Synonyms: CBSL; HIP4
• Gene ID: 875
• mRNA Refseq: NM_000071.3
• Protein Refseq: NP_000062.1
• MIM: 613381
• UniProt ID: P35520