||Recombinant Human CUL1(1-410aa) fused with His tag at N-terminal was expressed in E. coli.
||Cullin1, also known CuL1, is a core component of multiple cullin-RING-based SCF (SKP1-CuL1-F-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, it serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits. Cullin1 may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme.
||Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 100mM NaCl
||49.4 kDa (430aa) confirmed by MALDI-TOF
||MGSSHHHHHH SSGLVPRGSH MSSTRSQNPH GLKQIGLDQI WDDLRAGIQQ VYTRQSMAKS RYMELYTHVY NYCTSVHQSN QARGAGVPPSKSKKGQTPGG AQFVGLELYK RLKEFLKNYL TNLLKDGEDL MDESVLKFYT QQWEDYRFSS KVLNGICAYL NRHWVRRECD EGRKGIYEIYSLALVTWRDC LFRPLNKQVT NAVLKLIEKE RNGETINTRL ISGVVQSYVE LGLNEDDAFA KGPTLTVYKE SFESQFLADT ERFYTRESTEFLQQNPVTEY MKKAEARLLE EQRRVQVYLH ESTQDELARK CEQVLIEKHL EIFHTEFQNL LDADKNEDLG RMYNLVSRIQ DGLGELKKLL
ETHIHNQGLA AIEKCGEAAL NDPKMYVQTV LDVHKKYNAL VMSAFNNDAG FVAALDKACG RFINNNAVTK
||> 95% by SDS - PAGE
||Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
||1 mg/ml (determined by Bradford assay)