Human CUL1 Knockdown Cell Lysate

• Cat.No.: CUL1-009HKCL
• Product Overview: WB-validated CUL1 Knockdown 293T Cell Lysate

Specification

• Species: Human
• Source: 293T
• Tag: Non
• Description: Enables ubiquitin ligase complex scaffold activity and ubiquitin protein ligase binding activity. Involved in SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; positive regulation of canonical NF-kappaB signal transduction; and protein K48-linked ubiquitination. Located in plasma membrane. Part of Parkin-FBXW7-Cul1 ubiquitin ligase complex and SCF ubiquitin ligase complex.
• Form: Cell-Tissue Lysis buffer
• Molecular Mass: 90 kDa
• Notes: Instruction of use: This knockdown cell lysate should be paired with wild-type 293T cell lysate for use. For Western blotting, we recommend running wild-type and knockdown lysates on the same gel, and loading each well with equal volume and equal amount of total proteins.
• Storage: Store at -20 centigrade for two years.
• Concentration: Lot-specific
• Shipping: Blue Ice
• Components: 1 vial of 100 μg WT 293T cell lysate 1 vial of 100 μg CUL1 KD 293T cell lysate
• Protein Families: Druggable Genome
• Protein Pathways: Cell cycle, Oocyte meiosis, TGF-beta signaling pathway, Ubiquitin mediated proteolysis, Wnt signaling pathway
• Lysate QC: RT-qPCR; Western Blotting (WB)

Gene Information

• Gene Name: CUL1 cullin 1 [ Homo sapiens (human) ]
• Official Symbol: CUL1
• Synonyms: CUL1; cullin 1; cullin-1; CUL-1; MGC149834; MGC149835
• Gene ID: 8454
• mRNA Refseq: NM_003592
• Protein Refseq: NP_003583
• MIM: 603134
• UniProt ID: Q13616