Recombinant Human DHFR, His-tagged

• Cat.No.: DHFR-28331TH
• Product Overview: Recombinant full length Human Dihydrofolate reductase with an N terminal His tag; 207 amino acids inclusive of tag; predicted mwt: 23.8 kDa

Specification

• Description: Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase-like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia.
• Protein length: 187 amino acids
• Conjugation: HIS
• Molecular Weight: 23.800kDa inclusive of tags
• Source: E. coli
• Form: Liquid
• Purity: >95% by SDS-PAGE
• Storage buffer: Preservative: NoneConstituents: 10% Glycerol, 20mM Tris HCl, 2mM DTT, 100mM Sodium chloride, pH 8
• Storage: Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MVRPLNCIVA VSQNMGIGKN GDLPWPPLRN EFKYFQRMTT TSSVEGKQNL VIMGRKTWFS IPEKNRPLKD RINIVLSREL KEPPRGAHFL AKSLDDALRL IEQPELASKV DMVWIVGGSS VYQEAMNQPG HLRLFVTRIM QEFESDTFFP EIDLGKYKLL PEYPGVLSEV QEEKGIKYKF EVYEKKD
• Sequence Similarities: Belongs to the dihydrofolate reductase family.Contains 1 DHFR (dihydrofolate reductase) domain.

Gene Information

• Gene Name: DHFR dihydrofolate reductase [ Homo sapiens ]
• Official Symbol: DHFR
• Synonyms: DHFR; dihydrofolate reductase
• Gene ID: 1719
• mRNA Refseq: NM_000791
• Protein Refseq: NP_000782
• MIM: 126060
• Uniprot ID: P00374
• Chromosome Location: 5q11.2-q13.2
• Pathway: Cell Cycle, Mitotic, organism-specific biosystem; E2F mediated regulation of DNA replication, organism-specific biosystem; E2F transcription factor network, organism-specific biosystem; Fluoropyrimidine Activity, organism-specific biosystem; Folate biosynthesis, organism-specific biosystem