Recombinant Human GDNF

• Cat.No.: GDNF-26274TH
• Product Overview: Recombinant full length human GDNF containing 2 x 135 amino acid, 30,360 Dalton.

Specification

• Description: This gene encodes a highly conserved neurotrophic factor. The recombinant form of this protein was shown to promote the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosis of motor neurons induced by axotomy. The encoded protein is processed to a mature secreted form that exists as a homodimer. The mature form of the protein is a ligand for the product of the RET (rearranged during transfection) protooncogene. Multiple transcript variants encoding different isoforms have been found for this gene. Mutations in this gene may be associated with Hirschsprung disease.
• Source: E. coli
• Tissue specificity: In the brain, predominantly expressed in the striatum with highest levels in the caudate and lowest in the putamen.
• Form: Lyophilised:Reconstitute in sterile 18MO-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions.
• Purity: >95% by SDS-PAGE
• Storage buffer: Preservative: NoneConstituents: 150mM Sodium chloride, 10mM Sodium citrate
• Storage: Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
• Sequences of amino acids: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ser-Pro-Asp-Lys.
• Sequence Similarities: Belongs to the TGF-beta family. GDNF subfamily.
• Publication:
  Neurite Aggregation and Calcium Dysfunction in iPSC-Derived Sensory Neurons with Parkinson's Disease-Related LRRK2 G2019S Mutation. (2015)
  Sensory Neurons Do Not Induce Motor Neuron Loss in a Human Stem Cell Model of Spinal Muscular Atrophy (2014)

Gene Information

• Gene Name: GDNF glial cell derived neurotrophic factor [ Homo sapiens ]
• Official Symbol: GDNF
• Synonyms: GDNF; glial cell derived neurotrophic factor; glial cell line-derived neurotrophic factor; astrocyte derived trophic factor; ATF1; ATF2; glial cell line derived neurotrophic factor; glial derived neurotrophic factor; HFB1 GDNF
• Gene ID: 2668
• mRNA Refseq: NM_000514
• Protein Refseq: NP_000505
• MIM: 600837
• Uniprot ID: P39905
• Chromosome Location: 5p13.1-p12
• Pathway: Axon guidance, organism-specific biosystem; Developmental Biology, organism-specific biosystem; NCAM signaling for neurite out-growth, organism-specific biosystem; NCAM1 interactions, organism-specific biosystem; Signaling events regulated by Ret tyrosine kinase, organism-specific biosystem
• Function: growth factor activity; protein homodimerization activity; receptor binding