Recombinant Mouse Ephrin B2, Fc-His

• Cat.No.: Efnb2-1734M
• Product Overview: Recombinant Mouse EphrinB2 (Arg27-Ala227) produced inNSO Cellswas fused to Fc region of human IgG1 (Pro100 - Lys330) - 6xHis tag

Specification

• Species: Mouse
• Source: Mammalian Cells
• Tag: Fc&His
• Protein Length: 27-227 a.a.
• Description: Ephrin-B2 (EFNB2) is a transmembrane ligand that is specifically expressed on arterial endothelial cells (ECs) and surrounding cells and interacts with multiple EphB class receptors. Ephrin-B2 plays a role in angiogenesis and regulates the targeted migration of branchial neural crest cells. EFNB2 serves as a functional receptor for both Hendra virus (HeV) and Nipah virus (NiV).
• Molecular Weight: The predicted molecular weight of Recombinant Mouse Ephrin-B2 is Mr 49.6 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 60-65 kDa.
• State Of Matter: Lyophilized.
• Purity: >90% by SDS Page and analyzed by silver stain.
• Formulation: This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
• Endotoxin: <1.0 EU/µg as determined by the LAL method.
• Biological Activity: The biological activity of Mouse EFNB2 was determined by its ability to compete with biotinylated rmEphrinB2 for binding with immobilized rmEphB2 in a functional ELISA assay.
• Storage And Stability: This lyophilized protein is stable for six to twelve months when stored desiccated at -20℃ to -70℃. After aseptic reconstitution, this protein may be stored at 2℃ to 8℃ for one month or at -20℃ to -70℃ in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Gene Information

• Gene Name: Efnb2 ephrin B2 [ Mus musculus ]
• Synonyms: Efnb2; ephrin B2; Epl5; ELF-2; Eplg5; Htk-L; Lerk5; LERK-5; NLERK-1; OTTMUSP00000024973
• Gene ID: 13642
• mRNA Refseq: NM_010111
• Protein Refseq: NP_034241
• UniProt ID: P52800
• Chromosome Location: 8 A1.1; 8 2.0 cM
• Pathway: Axon guidance
• Function: receptor binding