Recombinant Human GPT, His-tagged
Cat.No. : | GPT-26390TH |
Product Overview : | Recombinant full-length Human Alanine Transaminase with a N terminal His tag. 516 amino acids with a predicted MWt 56.8 kDa including tag. |
- Specification
- Gene Information
- Related Products
Description : | This gene encodes cytosolic alanine aminotransaminase 1 (ALT1); also known as glutamate-pyruvate transaminase 1. This enzyme catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. A related gene on chromosome 16 encodes a putative mitochondrial alanine aminotransaminase. |
Protein length : | 496 amino acids |
Conjugation : | HIS |
Molecular Weight : | 56.800kDa inclusive of tags |
Source : | E. coli |
Tissue specificity : | Liver, kidney, heart, and skeletal muscles. Expressed at moderate levels in the adipose tissue. |
Form : | Liquid |
Purity : | >90% by SDS-PAGE |
Storage buffer : | pH: 8.00Constituents:0.32% Tris HCl, 20% Glycerol, 0.03% DTT |
Storage : | Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. |
Sequences of amino acids : | MGSSHHHHHHSSGLVPRGSHMASSTGDRSQAVRHGLRAKV LTLDGMNPRVRRVEYAVRGPIVQRALELEQELRQGVKK PFTEVIRANIGDAQAMGQRPITFLRQVLALCVNPDLLSSP NFPDDAKKRAERILQACGGHSLGAYSVSSGIQLIREDV ARYIERRDGGIPADPNNVFLSTGASDAIVTVLKLLVAGEG HTRTGVLIPIPQYPLYSATLAELGAVQVDYYLDEERAW ALDVAELHRALGQARDHCRPRALCVINPGNPTGQVQTREC IEAVIRFAFEERLFLLADEVYQDNVYAAGSQFHSFKKV LMEMGPPYAGQQELASFHSTSKGYMGECGFRGGYVEVVNM DAAVQQQMLKLMSVRLCPPVPGQALLDLVVSPPAPTDP SFAQFQAEKQAVLAELAAKAKLTEQVFNEAPGISCNPVQG AMYSFPRVQLPPRAVERAQELGLAPDMFFCLRLLEETG ICVVPGSGFGQREGTYHFRMTILPPLEKLRLLLEKLSRFH AKFTLEYS |
Sequence Similarities : | Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily. |
Gene Name : | GPT glutamic-pyruvate transaminase (alanine aminotransferase) [ Homo sapiens ] |
Official Symbol : | GPT |
Synonyms : | GPT; glutamic-pyruvate transaminase (alanine aminotransferase); alanine aminotransferase 1; ALT1; GPT1; |
Gene ID : | 2875 |
mRNA Refseq : | NM_005309 |
Protein Refseq : | NP_005300 |
MIM : | 138200 |
Uniprot ID : | P24298 |
Chromosome Location : | 8q24.2-qter |
Pathway : | Alanine and aspartate metabolism, organism-specific biosystem; Alanine, aspartate and glutamate metabolism, organism-specific biosystem; Alanine, aspartate and glutamate metabolism, conserved biosystem; Amino acid synthesis and interconversion (transamination), organism-specific biosystem; Metabolic pathways, organism-specific biosystem; |
Function : | 1-aminocyclopropane-1-carboxylate synthase activity; L-alanine:2-oxoglutarate aminotransferase activity; L-alanine:2-oxoglutarate aminotransferase activity; pyridoxal phosphate binding; transaminase activity; |
Products Types
◆ Recombinant Protein | ||
GPT-2330R | Recombinant Rat GPT Protein, His (Fc)-Avi-tagged | +Inquiry |
GPT-1015H | Recombinant Human GPT Protein, His (Fc)-Avi-tagged | +Inquiry |
GPT-2236H | Recombinant Human GPT Protein, MYC/DDK-tagged | +Inquiry |
GPT-5304H | Recombinant Human GPT Protein, GST-tagged | +Inquiry |
GPT-1785R | Recombinant Rhesus Macaque GPT Protein, His (Fc)-Avi-tagged | +Inquiry |
◆ Native Protein | ||
GPT-187H | Active Native Human Glutamate Pyruvate Transaminase | +Inquiry |
GPT-65H | Active Native Human Glutamate Pyruvate Transaminase (GPT) | +Inquiry |
GPT-26882TH | Native Human GPT | +Inquiry |
◆ Lysates | ||
GPT-721RCL | Recombinant Rat GPT cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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