Recombinant Human MGMT protein, His-tagged

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Recombinant Human MGMT protein, His-tagged

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Cat.No. : MGMT-28449TH
Product Overview : Recombinant Human MGMT(1-207aa) fused with His tag at N-terminal was expressed in E. coli.
Description : O-6-methylguanine-DNA methyltransferase (MGMT) is an enzyme that repairs O-6-methylguanine, a mutagenic DNA base damaged by endogenous and environmental alkylating agents and is involved in the cellular defense against the biological effects of O-6-methylguanine in DNA. MGMT repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. There are few reports that abnormal MGMT expression correlates with the prognosis in human solid cancers.
Source : E. coli
Species : Human
Tag : His
Form : Liquid. In 20 mM Tris-HCl buffer (pH 7.5) containing 1 mM DTT, 10% glycerol
Molecular Mass : 23.8 kDa (227 aa), confirmed by MALDI-TOF. (Molecular weight on SDS-PAGE will appear higher)
AA Sequence : MGSSHHHHHH SSGLVPRGSH MDKDCEMKRT TLDSPLGKLE LSGCEQGLHE IKLLGKGTSA ADAVEVPAPA AVLGGPEPLM QCTAWLNAYFHQPEAIEEFP VPAFHHPVFQ QESFTRQVLW KLLKVVKFGE VISYQQLAAL AGNPKAARAV GGAMRGNPVP ILIPCHRVVC SSGAVGNYSGGLAVKEWLLA HEGHRLGKPG LGGSSGLAGA WLKGAGATSG SPPAGRN
Endotoxin : < 1.0 EU per 1 microgram of protein (determined by LAL method)
Purity : > 95% by SDS - PAGE
Storage : Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
Concentration : 0.5 mg/ml (determined by Bradford assay)
Gene Name : MGMT O-6-methylguanine-DNA methyltransferase [ Homo sapiens ]
Official Symbol : MGMT
Synonyms : MGMT; O-6-methylguanine-DNA methyltransferase; methylated-DNA--protein-cysteine methyltransferase; methylguanine-DNA methyltransferase; O-6-methylguanine-DNA-alkyltransferase; O6-methylguanine-DNA methyltransferase; 6-O-methylguanine-DNA methyltransferase;
Gene ID : 4255
mRNA Refseq : NM_002412
Protein Refseq : NP_002403
MIM : 156569
UniProt ID : P16455
Chromosome Location : 10q26
Pathway : DNA Damage Reversal, organism-specific biosystem; DNA Repair, organism-specific biosystem;
Function : DNA binding; DNA-methyltransferase activity; calcium ion binding; methylated-DNA-[protein]-cysteine S-methyltransferase activity; methyltransferase activity; transferase activity;
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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