Recombinant Human TNFRSF11B

• Cat.No.: TNFRSF11B-187H
• Product Overview: Recombinant Human Osteoprotegerin encoding the mature human Osteoprotegerin protein sequence Met 1 - Leu 401 was inserted into a suitable expression vector and expressed inNSO cells.

Specification

• Cat. No.: TNFRSF11B-187H
• Description: Osteoprotegerin (OPG), also known as osteoclastogenesis inhibitory factor (OCIF) is a cytokine, which can inhibit osteoclast differentiation, activity, and survival. It is a member of the tumor necrosis factor (TNF) receptor superfamily. OPG may provide a pharmacological tool for osteoporotic and erosive bone disorders.
• Source: Mammalian cells
• State Of Matter: Lyophilized.
• Purity: >95% by SDS Page and analyzed by silver stain.
• Endotoxin: <1.0 EU/µg as determined by the LAL method.
• Biological Activity: The biological activity of Human OPG was determined by its ability to neutralize apoptosis of mouse L-929 cells treated with 20 ng/mL cross-linked soluble rhTRAIL. The expected ED50 for this effect is typically 8 - 24 ng/ml.
• Molecular Weight: The predicted molecular weight of each monomer of mature OPG (generated after removal of a 21 amino acid signal peptide) is Mr 43.5 kDa. However, the actual molecular weight as observed by migration on SDS Page under reducing conditions, as a result of glycosylation, of recombinant Human OPG is Mr 55 kDa.
• Formulation: This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
• Storage And Stability: This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

Gene Information

• Gene Name: TNFRSF11B tumor necrosis factor receptor superfamily, member 11b [ Homo sapiens ]
• Synonyms: TNFRSF11B, OPG, OCIF, Osteoclastogenesis inhibitory factor, TR1, MGC29565, Osteoprotegerin, TR1, Osteoclastogenesis inhibitory factor, tumor necrosis factor receptor superfamily, member 11b
• Gene ID: 4982
• mRNA Refseq: NM_002546
• Protein Refseq: NP_002537
• MIM: 602643
• UniProt ID: O00300
• Chromosome Location: 8q24
• Pathway: Cytokine-cytokine receptor interaction
• Function: cytokine activity; protein binding; receptor activity