Recombinant Human PLK3, His-tagged

• Cat.No.: PLK3-31032TH
• Product Overview: Recombinant fragment, corresponding to amino acids 58-339 of Human PLK3, with N terminal His tag.

Specification

• Description: Cytokine-inducible kinase is a putative serine/threonine kinase.CNK contains both a catalytic domain and a putative regulatory domain.It may play a role in regulation of cell cycle progression and tumorigenesis.
• Conjugation: HIS
• Tissue specificity: Transcripts are highly detected in placenta, lung, followed by skeletal muscle, heart, pancreas, ovaries and kidney and weakly detected in liver and brain. May have a short half-live. In cells of hematopoietic origin, strongly and exclusively detected in
• Biological activity: Activity: 500 U/mg. One unit is defined as the amount of enzyme that willphosphorylate 1 nmol of casein substrate per minute at pH 7.4 and 30C. Assay buffer: 50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 uM Na-orthovanadate, 0.5 mM ATP.
• Form: Liquid
• Storage buffer: Preservative: NoneConstituents: 50% Glycerol, 0.05% Tween 20, 3mM DTT, 25mM Tris HCl, 100mM Sodium chloride, pH 8.0
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Sequences of amino acids: SGR TYLKGRLLGK GGFARCYEAT DTETGSAYAV KVIPQSRVAK PHQREKILNE IELHRDLQHRHIVRFSHHFE DADNIYIFLE LCSRKSLAHI WKARHTLLEP EVRYYLRQIL SGLKYLHQRGILHRDLKLGN FFITENMELK VGDFGLAARL EPPEQRKKTI CGTPNYVAPE VLLRQGHGPEADVWSLGCVM YTLLCGSPPF ETADLKETYR CIKQVHYTLP ASLSLPARQL LAAILRASPRDRPSIDQILR HDFFTKGYTP DRLPISSCVT VPDLTPPNP
• Sequence Similarities: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.Contains 2 POLO box domains.Contains 1 protein kinase domain.

Gene Information

• Gene Name: PLK3 polo-like kinase 3 [ Homo sapiens ]
• Official Symbol: PLK3
• Synonyms: PLK3; polo-like kinase 3; CNK, cytokine inducible kinase , polo like kinase 3 (Drosophila); serine/threonine-protein kinase PLK3; FNK; PRK
• Gene ID: 1263
• mRNA Refseq: NM_004073
• Protein Refseq: NP_004064
• MIM: 602913
• Uniprot ID: Q9H4B4
• Chromosome Location: 1p34.1
• Pathway: Direct p53 effectors, organism-specific biosystem; PLK3 signaling events, organism-specific biosystem; Polo-like kinase signaling events in the cell cycle, organism-specific biosystem; Tuberculosis, organism-specific biosystem; Tuberculosis, conserved biosystem
• Function: ATP binding; nucleotide binding; p53 binding; protein binding; protein serine/threonine kinase activity