Recombinant Human POLR2E, His-tagged

• Cat.No.: POLR2E-28615TH
• Product Overview: Recombinant full length human His-tagged POLR2E isolated from an E. coli strain that carries the coding sequence of the protein under the control of a T7 promoter.

Specification

• Description: This gene encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11.
• Conjugation: HIS
• Source: E. coli
• Form: Liquid
• Purity: >95% by SDS-PAGE
• Storage buffer: Preservative: NoneConstituents: 20% Glycerol, 20mM Tris HCl, 100mM Potassium chloride, 1mM DTT, 0.2mM EDTA, pH 8.0
• Storage: Shipped on dry ice. Upon delivery aliquot and store at -80oC. Avoid freeze / thaw cycles.
• Sequence Similarities: Belongs to the archaeal rpoH/eukaryotic RPB5 RNA polymerase subunit family.

Gene Information

• Gene Name: POLR2E polymerase (RNA) II (DNA directed) polypeptide E, 25kDa [ Homo sapiens ]
• Official Symbol: POLR2E
• Synonyms: POLR2E; polymerase (RNA) II (DNA directed) polypeptide E, 25kDa; polymerase (RNA) II (DNA directed) polypeptide E (25kD); DNA-directed RNA polymerases I, II, and III subunit RPABC1; DNA directed RNA polymerase II 23 kda polypeptide; hRPB25; hsRPB5; RPABC1
• Gene ID: 5434
• mRNA Refseq: NM_002695
• Protein Refseq: NP_002686
• MIM: 180664
• Uniprot ID: P19388
• Chromosome Location: 19p13.3
• Pathway: Abortive elongation of HIV-1 transcript in the absence of Tat, organism-specific biosystem; Cytosolic DNA-sensing pathway, organism-specific biosystem; Cytosolic DNA-sensing pathway, conserved biosystem; DNA Repair, organism-specific biosystem; Dual incision reaction in TC-NER, organism-specific biosystem
• Function: DNA binding; DNA-directed RNA polymerase activity; contributes_to protein kinase activity