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Recombinant Mouse Insulin-like Growth Factor Binding Protein 2

Cat. No.: Igfbp2-1749M
Product Overview: Recombinant Mouse Igfbp2 encoding the full length (Met 1 - Gln 305) was inserted into a suitable mammalian expression vector and expressed inNSO cells.
Description: Insulin-like growth factor binding protein 2, also known as IGFBP2, is an extracellular unglycosylated polypeptide that specifically binds IGF and modulates its effects. IGFBP-2 has direct effects on breast cancer proliferation via stimulation of critical signaling pathways.
Molecular Weight: The predicted molecular weight of Recombinant Mouse IGFBP-2 is Mr 29.4 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 35 kDa.
Source: NSO Cells.
State Of Matter: Lyophilized.
Purity: >95% by SDS Page and analyzed by silver stain.
Formulation: This recombinant protein was lyophilized from a 0.2 μm filtered solution in 30% acetonitrile (CH3CN) and 0.1% trifluoroacetic acid (TFA).
Endotoxin: <1.0 EU/µg as determined by the LAL method.
Biological Activity: The biological activity of Mouse IGFBP-2 was determined by its ability to inhibit the biological activity of rmIGF-II on MCF-7 cells. The expected ED50 is typically 0.125 - 0.5 μg/ml in the presence of 30 ng/ml recombinant mouse IGF-II.
Storage And Stability: This lyophilized protein is stable for six to twelve months when stored desiccated at -20°Cto -70°C. After aseptic reconstitution, this protein may be stored at 2°Cto 8°Cfor one month or at -20°Cto -70°Cin a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Gene Name: Igfbp2 insulin-like growth factor binding protein 2 [ Mus musculus ]
Synonyms: Igfbp2; insulin-like growth factor binding protein 2; Igfbp-2; AI255832
Gene ID: 16008
mRNA Refseq: NM_008342
Protein Refseq: NP_032368
UniProt ID: P47877
Chromosome Location: 1 C3; 1 36.1 cM
Function: growth factor binding; insulin-like growth factor binding

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