||Pleiotrophin (heparin binding growth factor 8, neurite growth-promoting factor 1), also known as PTN is a growth, differentiation inducing cytokine, and neurite extension promoting polypeptide which is highly expressed in brain and in tissues derived from mesenchyme. PTN is produced by many different human cancers and stimulates tumor blood vessel formation when it is expressed in malignant cancer cells. It is expressed in many types of cancer, such as gliomas, melanomas, meningiomas, neuroblastomas, choriocarcinomas, leukemias and cancers of pancreas, prostate, stomach, colon, breast, ovaries and lungs.
||Sf21 insect cells.
||The predicted molecular weight of Recombinant Human PTN is 15.3 kDa. However, the actual molecular weight as observed by migration on SDS Page is 18 kDa.
|State Of Matter:
||This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
||>97% by SDS Page and analyzed by silver stain.
||<1.0 EU/µg as determined by the LAL method.
||The biological activity of Human PTN was determined by its ability to enhance neurite outgrowth of cerebral cortical neurons of E10 chick embryos (Hampton, B.S. et al., 1992, Mol. Biol. Cell 3:85 - 93). Optimal neurite outgrowth was observed when neurons were plated on 96 well culture plates that had been pre-coated with 100 μL/well of a solution of 3 - 8 μg/mL rhPTN.
||This lyophilized protein is stable for six to twelve months when stored desiccated at -20℃ to -70℃. After aseptic reconstitution, this protein may be stored at 2℃ to 8℃ for one month or at -20℃ to -70℃ in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.