||Recombinant Human SIRT1 (amino acids 193- 741) was expressed in anE.coliexpression system with N-terminal GST tag. MW=87.2 kDa.
||Sirtuin 1 also known as NAD-dependent deacetylase sirtuin-1 is a protein that in humans is encoded by the SIRT1 gene. SIRT1 stands for sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae), referring to the fact that its sirtuin homolog (biological equivalent across species) in yeast (S. cerevisiae) is Sir2. SIRT1 is an enzyme which deacetylates proteins that contribute to cellular regulation (reaction to stressors, longevity).
||25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20 and 20% glycerol.
||Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
||8 pmol/min /μg. Assay condition: 25 mM Tris/Cl, pH8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 30 μM Biomol substrate (Catalog number KI-177), 200 μM NAD+and 20 ng/μl Sirtuin 1. Incubation condition: 30 min at 30ºC.
||>6 months at –80°C.