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Recombinant Human SIRT1, GST-tagged

Cat. No.: SIRT1-462H
Product Overview: Recombinant Human SIRT1 (amino acids 193- 741) was expressed in anE.coliexpression system with N-terminal GST tag. MW=87.2 kDa.
Description: Sirtuin 1 also known as NAD-dependent deacetylase sirtuin-1 is a protein that in humans is encoded by the SIRT1 gene. SIRT1 stands for sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae), referring to the fact that its sirtuin homolog (biological equivalent across species) in yeast (S. cerevisiae) is Sir2. SIRT1 is an enzyme which deacetylates proteins that contribute to cellular regulation (reaction to stressors, longevity).
Source: E.Coli.
Formulated In: 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20 and 20% glycerol.
Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Specific Activity: 8 pmol/min /μg. Assay condition: 25 mM Tris/Cl, pH8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 30 μM Biomol substrate (Catalog number KI-177), 200 μM NAD+and 20 ng/μl Sirtuin 1. Incubation condition: 30 min at 30ºC.
Purity: > 80%.
Stability: >6 months at –80°C.
Gene Name: SIRT1 sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae) [ Homo sapiens ]
Synonyms: sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae); SIR2L1; SIRT1; sirtuin 1; SIR2alpha; sir2-like 1; sirtuin type 1; EC 3.5.1.-; OTTHUMP00000019691; OTTHUMP00000060745; SIR2alpha; hSIR2; hSIRT1; SIR2-like protein 1; sirtuin (silent mating type information regulation 2, S. cerevisiae, homolog)
Gene ID: 23411
mRNA Refseq: NM_001142498
Protein Refseq: NP_001135970
MIM: 604479
UniProt ID: Q96EB6
Chromosome Location: 10q21.3
Function: NAD binding; NAD+ ADP-ribosyltransferase activity; NAD-dependent histone deacetylase activity; deacetylase activity; histone binding; hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides; identical protein binding; metal ion binding; p53 binding; protein C-terminus binding; protein domain specific binding; transcription corepressor activity; zinc ion binding

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