||Recombinant Mouse Sphingosine kinase 2 (full length) was expressed in aBaculovirus infected Sf9cell expression system with N-terminal His tag. MW=68 kDa.
||Sphingosine kinase (SphK) is a conserved lipid kinase that catalyzes formation Sphingosine-1-phosphate (S1P) from the precursor sphingolipid sphingosine. Sphingolipid metabolites, such as ceramide, sphingosine and sphingosine-1-phosphate, are lipid second messengers involved in diverse cellular processes. There are two forms of SphK, SphK1 and SphK2. SPHK1 is found in the cytosol of eukaryotic cells, and migrates to the plasma membrane upon activation. SphK2 is localized to the nucleus.
||Baculovirus infected Sf9 cells.
||25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, and 3 mM DTT.
||Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
||2 U/ug. Sphingosine kinase activity was determined using D-erythrosphingosine and ATP as substrates. A unit of sphingosine kinase activity is defined as the amount of enzyme required to produce 1 pmol of S1P/min. Assay buffer: 50 mM HEPES, pH 7.4, 150 mM NaCl, 5 mM MgCl2, 1 mM DTT, 3 uM Na-orthovanadate, 0.5 mM ATP, 4 uM D-erythrosphingosine, and 5 ug/ml mouse sphingosine kinase 2.
||>70% by SDS-PAGE.
||> 6 months at –80°C.
||Calcium signaling pathway; Fc gamma R-mediated phagocytosis; Metabolic pathways; Sphingolipid metabolism; VEGF signaling pathway