||Recombinant His-tag fusion protein of KIR3DL1 cytoplasmic tail (361-444aa) was overexpressed as insoluble protein aggregates (132 aa, 15 kDa). This protein was purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a redox buffer.
||The three Ig-domain from of inhibitory killer cell Ig-like receptor 1(KIR3DL1,NKB1, nkat3, p70KIR) is a NK cell receptor for polymorphic HLA-B determinant. KIR3DLl recognizes the Bw4 determinant defined by sequence motifs at positins 77-83 of the HLA-B heavy chain. The cytoplasmic tail of KIR, which contains two immunoreceptor tyrosine-based inhibition motifs(ITIMs), mediates inhibitory signal transduction that prevents killer cell-mediated cytotoxicity.
||Liquid. In 25 mM Tris-HCl buffer (pH 7.5) containing 100 mM NaCl.
||15 kDa (132 aa).
||> 95% by SDS – PAGE.
||1 mg/ml (determined by Bradford assay).
||MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSTSGT IDKLDIEFHL WCSNKKNAAV MDQEPAGNRT ANSEDSDEQD PEEVTYAQLD HCVFTQRKIT RPSQRPKTPP TDTILYTELP NAKPRSKVVS CP
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.