Recombinant Human Killer Cell Immunoglobulin-like Receptor, Three Domains, Long Cytoplasmic Tail, 1, His-tagged

• Cat.No.: KIR3DL1-349H
• Product Overview: Recombinant His-tag fusion protein of KIR3DL1 cytoplasmic tail (361-444aa) was overexpressed as insoluble protein aggregates (132 aa, 15 kDa). This protein was purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a redox buffer.

Specification

• Cat. No.: KIR3DL1-349H
• Description: The three Ig-domain from of inhibitory killer cell Ig-like receptor 1(KIR3DL1,NKB1, nkat3, p70KIR) is a NK cell receptor for polymorphic HLA-B determinant. KIR3DLl recognizes the Bw4 determinant defined by sequence motifs at positins 77-83 of the HLA-B heavy chain. The cytoplasmic tail of KIR, which contains two immunoreceptor tyrosine-based inhibition motifs(ITIMs), mediates inhibitory signal transduction that prevents killer cell-mediated cytotoxicity.
• Source: E.coli.
• Formulation: Liquid. In 25 mM Tris-HCl buffer (pH 7.5) containing 100 mM NaCl.
• Molecular Weight: 15 kDa (132 aa).
• Purity: > 95% by SDS – PAGE.
• Concentration: 1 mg/ml (determined by Bradford assay).
• Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSTSGT IDKLDIEFHL WCSNKKNAAV MDQEPAGNRT ANSEDSDEQD PEEVTYAQLD HCVFTQRKIT RPSQRPKTPP TDTILYTELP NAKPRSKVVS CP
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: KIR3DL1
• Gene ID: 3811
• mRNA Refseq: NM_013289.2
• Protein Refseq: NP_037421.2
• MIM: 604946
• UniProt ID: P43629
• Chromosome Location: 19q13.4
• Pathway: Antigen processing and presentation; Natural killer cell mediated cytotoxicity; Signaling in Immune system.
• Function: HLA-B specific inhibitory MHC class I receptor activity; receptor activity.