Recombinant Swine Chemokine (C-C motif) Ligand 2

• Cat.No.: CCL2-144S
• Product Overview: Recombinant Swine CCL2 protein was expressed inYeastand purified by using Ion-exchange chromatography, 8.6 kDa (76 aa).

Specification

• Species: Pig
• Source: Yeast
• Tag: Non
• Description: Chemokine (C-C motif) ligand 2 (CCL2) is a small cytokine belonging to the CC chemokine family that is also known as monocyte chemotactic protein-1 (MCP-1). CCL2 recruits monocytes, memory T cells, and dendritic cells to sites of tissue injury and infection. This chemokine is produced as a protein precursor containing signal peptide of 23 amino acids and a mature peptide of 76 amino acids.
• Sequences Of Amino Acids: QPDAINSPVT CCYTLTSKKI SMQRLMSYRR VTSSKCPKEA VIFKTIAGKE ICAEPKQKWV QDSISHLDKK NQTPKP.
• Formulation/Reconstitution: Lyophilized. Reconstitute with sterile phosphate-buffered saline containing at least 0.1% carrier protein.
• Purity: >95% as visualized by SDS-PAGE analysis.
• Purification: Ion-exchange chromatography.
• Biological Activity: The biological activity of recombinant swine CCL2 was determined in a chemotaxis assay using a Boyden chamber with freshly isolated swine PBMCs using a Percoll gradient method. Bioactivity is typically detected at 30 ng/mL steadily, increasing through 1 ug/mL.
• Storage: Stable for up to twelve months from date of receipt at -20°C. Stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. Avoid repeated freeze/thaw cycles.
• Pathways: Chemokine signaling pathway; Cytokine-cytokine receptor interaction; NOD-like receptor signaling pathway

Gene Information

• Gene Name: CCL2 chemokine (C-C motif) ligand 2 [ Sus scrofa ]
• Synonyms: CCL2; chemokine (C-C motif) ligand 2; MCP-1;C-C motif chemokine 2; small-inducible cytokine A2; monocyte chemotactic protein 1;monocyte chemoattractant protein 1
• Gene ID: 397422
• mRNA Refseq: NM_214214
• Protein Refseq: NP_999379
• UniProt ID: P42831