Active Recombinant Human ADAMTS5, His-tagged
Cat.No. : | ADAMTS5-534H |
Product Overview : | Recombinant Human ADAMTS5 was expressed in abaculovirussystem and purified from insect cells. The enzyme contains the catalytic domain, the disintegrin domain and the thrombospondin type-1 motif of full-length ADAMTS-5 followed by a C-terminal His tag, 50 kDa. |
- Specification
- Gene Information
- Related Products
Cat. No. : | ADAMTS5-534H |
Description : | Aggrecanases belong to a family of ADAMTS proteases (ADAMTS = A disintegrin and metalloprotease with thrombospondin motifs). ADAMTS enzymes are comprised of an N-terminal prodomain, a catalytic domain, a disintegrin domain, one or more thrombospondin (TS) motifs, a cysteine-rich domain and a spacer domain of variable length. The prodomains are cleaved off intracellularly and the enzymes are processed further from their C-terminal ends after secretion. Aggrecanases are responsible for aggrecan degradation in osteoarthritic joints. ADAMTS-5 was found to be the principal aggrecanase in mouse cartilage. |
Source : | Baculovirus. |
Activity : | Activity (mU/mg) is specified on the vial. |
Purity : | ≥ 50%. |
Unit Definition : | One milliunit of protease hydrolyzes 1 nanomole of aggrecan interglobular domain substrate per minute at pH 7.5 at 37°C. |
Formulation : | 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 5 mM CaCl2, 50 mM Imidazol, 0.05% Brij-35. |
Application : | Used as a positive control in FRET-based and aggrecan enzyme activity assays, Western blot, IP, and ELISA. |
Storage : | Store at -80°C. Avoid multiple thaw-freeze cycles. |
Gene Name : | ADAMTS5 ADAM metallopeptidase with thrombospondin type 1 motif, 5 [ Homo sapiens ] |
Synonyms : | ADAM metallopeptidase with thrombospondin type 1 motif, 5; ADMP-2; ADAMTS11; FLJ36738; ADAMTS5; a disintegrin and metalloproteinase with thrombospondin motifs 5; ADAM-TS5; ADAM-TS 5; ADAMTS-11;ADAM-TS 11; aggrecanase-2; a disintegrin and metalloproteinase with thrombospondin motifs 11; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 5 (aggrecanase-2); EC 3.4.24.- |
Gene ID : | 11096 |
mRNA Refseq : | NM_007038 |
Protein Refseq : | NP_008969 |
MIM : | 605007 |
Chromosome Location : | 21q21.3 |
Function : | integrin binding; metal ion binding; metalloendopeptidase activity; metallopeptidase activity; peptidase activity; zinc ion binding |
Products Types
◆ Recombinant Protein | ||
ADAMTS5-144H | Active Recombinant Human ADAMTS5 Protein, His-tagged | +Inquiry |
ADAMTS5-2037M | Recombinant Mouse ADAMTS5 Protein (262-930 aa), His-tagged | +Inquiry |
ADAMTS5-2425H | Recombinant Human ADAMTS5 Protein, His (Fc)-Avi-tagged | +Inquiry |
ADAMTS5-1755H | Recombinant Human ADAMTS5 protein, His & T7-tagged | +Inquiry |
ADAMTS5-8483Z | Recombinant Zebrafish ADAMTS5 | +Inquiry |
◆ Lysates | ||
ADAMTS5-9028HCL | Recombinant Human ADAMTS5 293 Cell Lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Inquiry
0
Inquiry Basket