|Cat. No. :
|Product Overview :
||Recombinant Human ADAMTS5 was expressed in abaculovirussystem and purified from insect cells. The enzyme contains the catalytic domain, the disintegrin domain and the thrombospondin type-1 motif of full-length ADAMTS-5 followed by a C-terminal His tag, 50 kDa.
||Aggrecanases belong to a family of ADAMTS proteases (ADAMTS = A disintegrin and metalloprotease with thrombospondin motifs). ADAMTS enzymes are comprised of an N-terminal prodomain, a catalytic domain, a disintegrin domain, one or more thrombospondin (TS) motifs, a cysteine-rich domain and a spacer domain of variable length. The prodomains are cleaved off intracellularly and the enzymes are processed further from their C-terminal ends after secretion. Aggrecanases are responsible for aggrecan degradation in osteoarthritic joints. ADAMTS-5 was found to be the principal aggrecanase in mouse cartilage.
||Activity (mU/mg) is specified on the vial.
|Unit Definition :
||One milliunit of protease hydrolyzes 1 nanomole of aggrecan interglobular domain substrate per minute at pH 7.5 at 37°C.
||50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 5 mM CaCl2, 50 mM Imidazol, 0.05% Brij-35.
||Used as a positive control in FRET-based and aggrecan enzyme activity assays, Western blot, IP, and ELISA.
||Store at -80°C. Avoid multiple thaw-freeze cycles.