Recombinant Human Cystatin C


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Recombinant Human Cystatin C

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Cat. No. : CST3-1914H
Product Overview : Recombinant Human Cystatin C was expressed inhuman 293 cellsas a monomer with an apparent molecular mass of 12 to 13 kDa.
Description : Native Cystatin C in human urine is found in two different forms: one with pI 9.2 and the other with pI 7.8 by elimination of small basic peptides or amino acids from the N-terminal end of protein. Cystatin C has been studied for its role in predicting newonset or deteriorating cardiovascular disease. This cytokine is produced in a serum-free, chemically defined media.
Source : Human 293 cells.
Activity : 0.3 to 1.5 μM IC50.
Purity : >95%.
Endotoxin : Endotoxin-free.
Molecular Mass : 12 to 13 kDa, monomer, nonglycosylated.
Purity Confirmation : The protein was resolved by SDS polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.
Activity Assay : The inhibitory function of Cystatin C on papin's protease activity was measured by acolorimetric assay using L-BAPA as substrate. IC50 value was measured at 5 to 20 μg/mL (0.3 to 1.5 μM) with a range of 1.56μg/mL to 50μg/mL Cystatin C in presence of 0.55μM papain and 0.44μM L-BAPA.
PDB rendering based on 1r4c. :

Gene Name : CST3 cystatin C [ Homo sapiens ]
Synonyms : CST3; cystatin C; ARMD11; MGC117328; CST3; cystatin C (amyloid angiopathy and cerebral hemorrhage); cystatin C1; cystatin 3; Neuroendocrine basic polypeptide2; OTTHUMP000001641822; ARMD112; post-gamma-globulin; bA218C14.4 (cystatin C); OTTHUMP00000164181; Gamma-trace; gamma-trace; Post-gamma-globulin; MGC117328; Cystatin-3
Gene ID : 1471
mRNA Refseq : NM_000099
Protein Refseq : NP_000090
MIM : 604312
UniProt ID : P01034
Chromosome Location : 20p11.21
Function : beta-amyloid binding; cysteine-type endopeptidase inhibitor activity; peptidase inhibitor activity; protease binding; protein binding
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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