Recombinant Human Cystatin C

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Recombinant Human Cystatin C

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Cat. No. : CST3-1914H
Product Overview : Recombinant Human Cystatin C was expressed inhuman 293 cellsas a monomer with an apparent molecular mass of 12 to 13 kDa.
Description : Native Cystatin C in human urine is found in two different forms: one with pI 9.2 and the other with pI 7.8 by elimination of small basic peptides or amino acids from the N-terminal end of protein. Cystatin C has been studied for its role in predicting newonset or deteriorating cardiovascular disease. This cytokine is produced in a serum-free, chemically defined media.
Source : Human 293 cells.
Activity : 0.3 to 1.5 μM IC50.
Purity : >95%.
Endotoxin : Endotoxin-free.
Molecular Mass : 12 to 13 kDa, monomer, nonglycosylated.
Purity Confirmation : The protein was resolved by SDS polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.
Activity Assay : The inhibitory function of Cystatin C on papin's protease activity was measured by acolorimetric assay using L-BAPA as substrate. IC50 value was measured at 5 to 20 μg/mL (0.3 to 1.5 μM) with a range of 1.56μg/mL to 50μg/mL Cystatin C in presence of 0.55μM papain and 0.44μM L-BAPA.
PDB rendering based on 1r4c. :

Gene Name : CST3 cystatin C [ Homo sapiens ]
Synonyms : CST3; cystatin C; ARMD11; MGC117328; CST3; cystatin C (amyloid angiopathy and cerebral hemorrhage); cystatin C1; cystatin 3; Neuroendocrine basic polypeptide2; OTTHUMP000001641822; ARMD112; post-gamma-globulin; bA218C14.4 (cystatin C); OTTHUMP00000164181; Gamma-trace; gamma-trace; Post-gamma-globulin; MGC117328; Cystatin-3
Gene ID : 1471
mRNA Refseq : NM_000099
Protein Refseq : NP_000090
MIM : 604312
UniProt ID : P01034
Chromosome Location : 20p11.21
Function : beta-amyloid binding; cysteine-type endopeptidase inhibitor activity; peptidase inhibitor activity; protease binding; protein binding

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