||Recombinant Human Cystatin C was expressed inhuman 293 cellsas a monomer with an apparent molecular mass of 12 to 13 kDa.
||Native Cystatin C in human urine is found in two different forms: one with pI 9.2 and the other with pI 7.8 by elimination of small basic peptides or amino acids from the N-terminal end of protein. Cystatin C has been studied for its role in predicting newonset or deteriorating cardiovascular disease. This cytokine is produced in a serum-free, chemically defined media.
||Human 293 cells.
||0.3 to 1.5 μM IC50.
||12 to 13 kDa, monomer, nonglycosylated.
||The protein was resolved by SDS polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.
||The inhibitory function of Cystatin C on papin's protease activity was measured by acolorimetric assay using L-BAPA as substrate. IC50 value was measured at 5 to 20 μg/mL (0.3 to 1.5 μM) with a range of 1.56μg/mL to 50μg/mL Cystatin C in presence of 0.55μM papain and 0.44μM L-BAPA.