||Recombinant Rat TrkA was expressed inNSOCells.The recombinant mature human Trk A/Fc is a disulfide-linked homodimeric protein, Based on N-terminal.
||A DNA sequence encoding the signal peptide from human CD33 joined with the extracellular domain of human TrkA was fused to the Fc region of human IgG1 via a polypeptide linker.
||> 95%, as determined by SDS-PAGE and visualized by silver stain.
||< 1.0 EU per 1 μg of the enzyme as determined by the LAL method. Measured by its ability to inhibit NGF-induced proliferation of TF1 cells. The ED50 for this effect is typically 0.8-4 μg/mL in the presence of 10 ng/mL of rhNGF. The cell number is assessed in a fluorometric assay using a redox sensitive dye,
||Lyophilized from a 0.2 μm filtered solution in PBS.
||It is recommended that sterile PBS containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of no less than 100 μg/mL.
||Lyophilized samples are stable for up to six months at -20℃to -70℃. Upon reconstitution, this cytokine, in the presence of a carrier protein, can be stored under sterile conditions at 2 -8℃ for one month or at -20℃ to -70℃in a manual defrost freezer for three months without detectable loss of activity. Avoid repeated freeze-thaw cycles.