||Recombinant Mouse Alox12 fused with N-terminal his-tagged protein, was expressed using thebaculovirussystem. MW = 76.4 kDa.
||12-Lipoxygenase(12-LO) catalyzes the formation of 12-HpETE from arachidonic acid. There are two main types of 12-LO, platelet-type and leukocyte-type, which share only 60% identity at the amino acid level and strongly differ in substrate specificity and enzyme kinetics. Leukocyte-type 12-LO has the ability to also produce 15-HpETE, however, platelet-type predominately forms 12-HpETE. Epidermal-type 12(S)- and 12(R)-LO enzymes have also been characterized, which catalyze the synthesis of their respective stereospecific 12-HETE products.
||50 mM sodium phosphate, pH 7.2, containing 100 mM sodium chloride and 20% glycerol.
||≥6 months at -80°C; avoid freeze/thaw cycles by aliquoting the protein and storing at -80°C.