Recombinant mouse Alox12 Protein, His-tagged


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Recombinant mouse Alox12 Protein, His-tagged

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Cat.No. : Alox12-021M
Product Overview : Recombinant mouse Alox12 protein (2-664) with a C-terminal His-tag was expressed in insect cells
Description : This gene is from arachidonic acid. There are two main types of 12-LO, platelet-type and leukocyte-type, which share only 60% identity at the amino acid level and strongly differ in substrate specificity and enzyme kinetics. Leukocyte-type 12-LO has the ability to also produce 15-HpETE, however, platelet-type predominately forms 12-HpETE. 2 Epidermal-type 12(S)- and 12(R)-LO enzymes have also been characterized, which catalyze the synthesis of their respective stereospecific 12-HETE products.
Source : Insect Cells
Species : Mouse
Tag : His
Molecular Mass : 76 kDa
Protein length : 2-664
Purity : 80% estimated by SDS-PAGE
Unit Definition : One unit is defined as the amount of enzyme required to consume 1 nmol of oxygen per minute at 37 centigrade in 0.1 M Tris-HCl, pH 7.5, containing 5 mM EDTA, 0.03% polysorbate, and 100 µM arachidonate.
Stability : ≥ 1 year
Storage : -80 centigrade
Storage Buffer : 50 mM sodium phosphate pH 7.2, 100 mM sodium chloride, 20 µM FeCl2, and 30% glycerol
Shipping : Dry ice in continental US, may vary elsewhere.
SDS-PAGE analysis of Alox12 :

Lane 1: MW Markers
Lane 2: Purified 12-LO (2 µg)
Lane 3: Purified 12-LO (5 µg)

Gene Name : Alox12 arachidonate 12-lipoxygenase [ Mus musculus (house mouse) ]
Official Symbol : Alox12
Synonyms : Alox12; arachidonate 12-lipoxygenase; P-12LO; Alox12p; 9930022G08Rik; polyunsaturated fatty acid lipoxygenase ALOX12; 12S-LOX; 12S-lipoxygenase; arachidonate (12S)-lipoxygenase; arachidonate (15S)-lipoxygenase; arachidonate 12-lipoxygenase, 12S-type; arachidonate 15-lipoxygenase,15S-type; linoleate 13S-lipoxygenase; lipoxin synthase 12-LO; platelet-type lipoxygenase 12; EC; EC
Gene ID : 11684
mRNA Refseq : NM_001331118
Protein Refseq : NP_001318047
UniProt ID : P39655
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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