Active Recombinant Human SP1, HA & Flag-tagged
Cat.No. : | SP1-2093H |
Product Overview : | Recombinant Human SP1, fused to a HA- and a FLAG-tag, was expressed in insect cells (Schneider SL2 cells). |
- Specification
- Gene Information
- Related Products
Description : | The protein encoded by this gene is a zinc finger transcription factor that binds to GC-rich motifs of many promoters. The encoded protein is involved in many cellular processes, including cell differentiation, cell growth, apoptosis, immune responses, response to DNA damage, and chromatin remodeling. Post-translational modifications such as phosphorylation, acetylation, glycosylation, and proteolytic processing significantly affect the activity of this protein, which can be an activator or a repressor. Three transcript variants encoding different isoforms have been found for this gene. |
Source : | Insect cells |
Species : | Human |
Tag : | HA & Flag |
Form : | Liquid. In 50mM TRIS-HCl, pH 7.9, containing 420mM KCl, 0.1% NP-40, 12.5mM MgCl2 and 20% glycerol. |
Bio-activity : | 0.5BFU/μl (band forming units). 1BFU is sufficient to generate a strong band shift with a labelled oligonucleotide. |
Applications : | EMSA promoter characterization, analysis of nuclear extracts (oligonucleotides and recombinant protein serve as a positive control). |
Quality Control Test : | Sufficient for performing at least 25 protein-DNA interaction experiments in 10mM HEPES, pH 7.9, 0.1mM EDTA, 8.5% glycerol, 170mM KCl, 1μM ZnSO4, 37.5μg/ml Poly dIdC, 10mM DTT and 1mg/ml BSA, analyzed by gel shift assay. |
Storage : | 80oC. Avoid freeze/thaw cycles. |
Gene Name : | SP1 Sp1 transcription factor [ Homo sapiens (human) ] |
Official Symbol : | SP1 |
Synonyms : | SP1; Sp1 transcription factor; Sp1 transcription factor; specificity protein 1; TSFP1; Transcription factor Sp1 |
Gene ID : | 6667 |
mRNA Refseq : | NM_003109 |
Protein Refseq : | NP_003100 |
MIM : | 189906 |
UniProt ID : | P08047 |
Chromosome Location : | 12q13.1 |
Pathway : | Huntington"s disease; TGF-beta signaling pathway |
Function : | RNA polymerase II transcription factor activity; double-stranded DNA binding; histone deacetylase binding; metal ion binding; promoter binding; protein C-terminus binding; protein homodimerization activity; transcription activator activity; transcription factor activity; zinc ion binding |
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Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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