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|Product Overview :
||Recombinant Human Sp3 Transcription Factor along with FLAG tag is expressed inSL2 cells. The molecular weight of the recombinant human Sp3 is 81.9 kDa and contains 781amino acids.
||SP3 gene belongs to a family of Sp1 related genes that encode transcription factors that regulate transcription by binding to consensus GC- and GT-box regulatory elements in target genes. This protein contains a zinc finger DNA-binding domain and several transactivation domains, and has been reported to function as a bifunctional transcription factor that either stimulates or represses the transcription of numerous genes. Transcript variants encoding different isoforms have been described for this gene, and one has been reported to initiate translation from a non-AUG (AUA) start codon. Additional isoforms, resulting from the use of alternate downstream translation initiation sites, have also been noted. A related pseudogene has been identified on chromosome 13.
||> 50% by SDS-PAGE.
||>0.5 BFU/µl (band forming units) 1BFU is sufficient to generate a strong band shift with a labelled oligonucleotide.
||Sufficient for performing at least 25 protein-DNA interaction experiments in 10 mM HEPES (pH 7.9), 0.1 mM EDTA, 8.5 % Glycerol, 170 mM KCl, 1 µM ZnSO4, 37.5 µg/ml Poly dIdC, 10 mM DTT, and 1 mg/ml BSA, analyzed by gel shift assay.
||Liquid. In 20mM TRIS-Cl, pH 7.9, containing 150mM KCl, 0.2mM EDTA and 20% glycerol.
||Binds specifically to an oligonucleotide with the sequence: GGG GCG GGG.