||Recombinant Human NOS2 was produced inE.coli.MW = ~80kDa.
||Nitric oxide (NO) is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter; it is implicated in neurotoxicity associated with stroke and neurodegenerative diseases, neural regulation of smooth muscle, including peristalsis, and penile erection. NO is also responsible for endothelium-derived relaxing factor activity regulating blood pressure. In macrophages, NO mediates tumoricidal and bactericidal actions, as indicated by the fact that inhibitors of NO synthase (NOS) block these effects. Neuronal NOS and macrophage NOS are distinct isoforms. Both the neuronal and the macrophage forms are unusual among oxidative enzymes in requiring several electron donors: FAD, flavin mononucleotide (FMN), NADPH, and tetrahydrobiopterin.
||≥90% (SDS-PAGE and heme spectral analysis).
||Liquid. In 40mM bis-tris-propane, pH 7.8, containing 150mM sodium chloride and 0.1% BSA.
||≥35nmol/mg/mol protein. Amount assayed 150–500nM. Optimal conditions: 37°C, 40mM EPPS buffer, pH 7.6, 1mM NOHA, 0.5mM DTT, 30mM H2O2, 10units/ml superoxide dismutase, 0.5mg/ml BSA and variable concentrations of tetrahydrobioterin (0–1"000mM) in a final volume of 100ml. Reactions were initiated by 30mM H2O2 and stopped by 1"500 units of catalase.
||Stable for at least 1 year after receipt when stored at -80°C. Keep stock vial on ice at all times! Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C.