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Recombinant Human CARM1, His-tagged

Cat. No.: CARM1-617H
Product Overview: Recombinant Human CARM1 is produced inSF21 cellswith a His-tag.
Description: CARM1 (coactivator-associated arginine methyltransferase 1) is an enzyme encoded by the CARM1 gene found in human beings, as well as many other mammals. CARM1 is a secondary coactivator through its association with p160 family (SRC-1, GRIP1, AIB) of coactivators. It is responsible for moving cells toward the inner cell mass in developing blastocysts. This gene also plays an important role in androgen receptors and may play a role in prostate cancer progression.
Source: Sf21 Cells.
Purity: ≥90% (SDS-PAGE).
Concentration: 0.5mg/ml.
Formulation: Liquid. In 50mM TRIS-HCl, pH 7.5, containing 100mM sodium chloride, 0.2% NP-40, 50mM imidazole and 10% glycerol.
Endotoxin Content: <0.1EU/µg purified protein (LAL test; Bio Whittaker).
Specificity: ~50-100ng are required for standard HAT assay.
Storage And Stability: Stable for at least 6 months after receipt when stored at -80°C.
Gene Name: CARM1 coactivator-associated arginine methyltransferase 1 [ Homo sapiens ]
Synonyms: CARM1; coactivator-associated arginine methyltransferase 1; PRMT4; EC 2.1.1.125; 3` partial; protein arginine N-methyltransferase 4; Histone-arginine methyltransferase CARM1; EC 2.1.1.-; Protein arginine N-methyltransferase 4; Coactivator-associated arginine methyltransferase 1
Gene ID: 10498
mRNA Refseq: NM_199141
Protein Refseq: NP_954592
MIM: 603934
UniProt ID: Q86X55
Chromosome Location: 19p13.2
Pathway: Metabolism of lipids and lipoproteins
Function: histone-arginine N-methyltransferase activity; transferase activity; protein binding; transcription coactivator activity; protein methyltransferase activity

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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