Recombinant Human Early Growth Response 1

• Cat.No.: EGR1-2120H
• Product Overview: Recombinant Human Early Growth Response 1 was produced inSf9 cells.

Specification

• Cat. No.: EGR1-2120H
• Description: The recombinant EGR-1 protein can be employed in the following fields of research: a) Promoter studies: Identification of EGR-1 binding promoter elements. b) Detection of EGR-1 interacting proteins. c) Signal transduction: Assay for the effect on the nuclear component EGR-1. d) Screening for EGR-1 activity in tumor cells and lines. e) Drug screening: Effect on EGR-1 generation and DNA binding.
• Source: Sf9 cells.
• Formulation: Liquid. In 20mM HEPES, pH 7.9, containing 25% glycerol, 420mM sodium chloride, 0.2mM EDTA and 1.5mM magnesium chloride.
• Quantity: 50µl, corresponding to >25 band forming units (BFU). Sufficient for performing at least 25 gel shift assays under standard conditions.
• Specific Activity: >0.5BFU/µl. 1BFU is sufficient to generate a band shift with a labelled oligonucleotide.
• Specificity: Binds specifically to an oligonucleotide with the sequence GCG GGG GCG.
• Application: EMSA promoter characterization, in vitro transcription assays, analysis of nuclear extracts (oligonucleotides and recombinant protein serve as a positive control).
• Storage: Store at -80℃. Avoid freeze/thaw cycles.

Gene Information

• Gene Name: EGR1 early growth response 1 [ Homo sapiens ]
• Synonyms: EGR1; early growth response 1; TIS8; AT225; G0S30; NGFI-A; ZNF225; KROX-24; ZIF-268; EGR-1; zinc finger protein 225; transcription factor ETR103; transcription factor Zif268; zinc finger protein Krox-24; early growth response protein 1; nerve growth factor-induced protein A; KROX24;Protein Krox-243
• Gene ID: 1958
• mRNA Refseq: NM_001964
• Protein Refseq: NP_001955
• MIM: 128990
• UniProt ID: P18146
• Chromosome Location: 5q31.1
• Pathway: Prion diseases
• Function: DNA binding; double-stranded DNA binding; metal ion binding; protein binding; sequence-specific DNA binding; transcription activator activity; transcription factor activity; zinc ion binding

Reviews

02/25/2020

Perfect for cell assays.

10/07/2019

Great in biochemical studies.

11/23/2018

High quality, great performance.

Q&As

What therapeutic strategies are being explored targeting EGR1 in various diseases?

Therapeutic strategies targeting EGR1 are being explored in cancer, neurological disorders, and cardiovascular diseases, aiming to modulate its activity for therapeutic benefit.

How is dysregulation of EGR1 associated with cancer?

Dysregulation of EGR1 has been associated with various cancers, where it can function as either a tumor suppressor or oncogene depending on context.

What role does EGR1 play in cellular proliferation and differentiation?

EGR1 promotes cellular proliferation and plays a role in the initiation of cellular differentiation.

What is the main function of EGR1 in cellular processes?

EGR1 functions as a transcription factor, regulating the expression of genes involved in cell growth, differentiation, and response to stimuli.

How is EGR1 implicated in the cellular stress response?

EGR1 is a critical component of the immediate-early response to cellular stress, aiding in the activation of genes required for survival and repair.

In what way does EGR1 contribute to synaptic plasticity and learning?

In the nervous system, EGR1 is involved in synaptic plasticity, contributing to learning and memory processes.

How is EGR1 expression regulated in response to external stimuli?

EGR1 expression is rapidly induced in response to a variety of external signals, including growth factors, stress, and hormonal stimuli.