Recombinant Human Early Growth Response 1

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Recombinant Human Early Growth Response 1

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Cat. No. : EGR1-2120H
Product Overview : Recombinant Human Early Growth Response 1 was produced inSf9 cells.
Description : The recombinant EGR-1 protein can be employed in the following fields of research: a) Promoter studies: Identification of EGR-1 binding promoter elements. b) Detection of EGR-1 interacting proteins. c) Signal transduction: Assay for the effect on the nuclear component EGR-1. d) Screening for EGR-1 activity in tumor cells and lines. e) Drug screening: Effect on EGR-1 generation and DNA binding.
Source : Sf9 cells.
Formulation : Liquid. In 20mM HEPES, pH 7.9, containing 25% glycerol, 420mM sodium chloride, 0.2mM EDTA and 1.5mM magnesium chloride.
Quantity : 50µl, corresponding to >25 band forming units (BFU). Sufficient for performing at least 25 gel shift assays under standard conditions.
Specific Activity : >0.5BFU/µl. 1BFU is sufficient to generate a band shift with a labelled oligonucleotide.
Specificity : Binds specifically to an oligonucleotide with the sequence GCG GGG GCG.
Application : EMSA promoter characterization, in vitro transcription assays, analysis of nuclear extracts (oligonucleotides and recombinant protein serve as a positive control).
Storage : Store at -80℃. Avoid freeze/thaw cycles.
Cartoon representation of Zif268 (blue) containing three zinc fingers in complex with DNA (orange). The coordinating amino acid residues of the middle zinc ion (green) are highlighted. :

Gene Name : EGR1 early growth response 1 [ Homo sapiens ]
Synonyms : EGR1; early growth response 1; TIS8; AT225; G0S30; NGFI-A; ZNF225; KROX-24; ZIF-268; EGR-1; zinc finger protein 225; transcription factor ETR103; transcription factor Zif268; zinc finger protein Krox-24; early growth response protein 1; nerve growth factor-induced protein A; KROX24;Protein Krox-243
Gene ID : 1958
mRNA Refseq : NM_001964
Protein Refseq : NP_001955
MIM : 128990
UniProt ID : P18146
Chromosome Location : 5q31.1
Pathway : Prion diseases
Function : DNA binding; double-stranded DNA binding; metal ion binding; protein binding; sequence-specific DNA binding; transcription activator activity; transcription factor activity; zinc ion binding
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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