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Recombinant Human Early Growth Response 1

Cat. No.: EGR1-2120H
Product Overview: Recombinant Human Early Growth Response 1 was produced inSf9 cells.
Description: The recombinant EGR-1 protein can be employed in the following fields of research: a) Promoter studies: Identification of EGR-1 binding promoter elements. b) Detection of EGR-1 interacting proteins. c) Signal transduction: Assay for the effect on the nuclear component EGR-1. d) Screening for EGR-1 activity in tumor cells and lines. e) Drug screening: Effect on EGR-1 generation and DNA binding.
Source: Sf9 cells.
Formulation: Liquid. In 20mM HEPES, pH 7.9, containing 25% glycerol, 420mM sodium chloride, 0.2mM EDTA and 1.5mM magnesium chloride.
Quantity: 50µl, corresponding to >25 band forming units (BFU). Sufficient for performing at least 25 gel shift assays under standard conditions.
Specific Activity: >0.5BFU/µl. 1BFU is sufficient to generate a band shift with a labelled oligonucleotide.
Specificity: Binds specifically to an oligonucleotide with the sequence GCG GGG GCG.
Application: EMSA promoter characterization, in vitro transcription assays, analysis of nuclear extracts (oligonucleotides and recombinant protein serve as a positive control).
Storage: Store at -80℃. Avoid freeze/thaw cycles.
Gene Name: EGR1 early growth response 1 [ Homo sapiens ]
Synonyms: EGR1; early growth response 1; TIS8; AT225; G0S30; NGFI-A; ZNF225; KROX-24; ZIF-268; EGR-1; zinc finger protein 225; transcription factor ETR103; transcription factor Zif268; zinc finger protein Krox-24; early growth response protein 1; nerve growth factor-induced protein A; KROX24;Protein Krox-243
Gene ID: 1958
mRNA Refseq: NM_001964
Protein Refseq: NP_001955
MIM: 128990
UniProt ID: P18146
Chromosome Location: 5q31.1
Pathway: Prion diseases
Function: DNA binding; double-stranded DNA binding; metal ion binding; protein binding; sequence-specific DNA binding; transcription activator activity; transcription factor activity; zinc ion binding

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