Recombinant Human GPC3 protein (S359F), His-tagged, low endotoxin
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|Cat.No. :||GPC3-2246H Optional Service: Optional requirements on this protein|
|Product Overview :||Recombinant Human GPC3 (S359F, Gln25-His559)(low endotoxin) protein was fused to His-tag at C-terminus and expressed in human 293 cells (HEK293).|
|Description :||Glypican-3 (GPC3) is also known as Intestinal protein OCI-5, GTR2-2, MXR7, which belongs to the glypican family. Glypican 3/GPC-3 is highly expressed in lung, liver and kidney. Glypican-3 inhibits the dipeptidyl peptidase activity of DPP4. Glypican-3 may be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs, and also may play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function.|
|Predicted N Terminal :||Gln25|
|Form :||Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.|
|Molecular Mass :||The protein has a calculated MW of 61.7 kDa. The protein migrates as 66-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.|
|Protein length :||Gln25-His559|
|Endotoxin :||Less than 0.1 EU per μg by the LAL method.|
|Purity :||>90% as determined by SDS-PAGE.|
|Storage :||For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
|Reconstitution :||It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.|
|SDS-PAGE of GPC3-2246H :||
Human Glypican 3 (S359F), His Tag, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
|Bioactivity-ELISA of GPC3-2246H :||
Immobilized Human FGF basic, Tag Free at 0.5 μg/mL (100 μL/well) can bind Human Glypican 3 (S359F), His Tag, low endotoxin with a linear range of 3-128 ng/mL (QC tested).
Immobilized Human Glypican 3 (S359F), His Tag, low endotoxin at 0.5 μg/mL (100 μL/well) can bind Anti-Human GPC3 Mab with a linear range of 0.4-3 ng/mL (Routinely tested).
|Bioactivity-SPR of GPC3-2246H :||
Anti-Human GPC3 MAb (chimeric mouse-human IgG1) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human Glypican 3 (S359F), His Tag, low endotoxin with an affinity constant of 1.96 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
|Bioactivity-BLI of GPC3-2246H :||
Loaded Anti-Human GPC3 MAb (chimeric mouse-human IgG1) on AHC Biosensor, can bind Human Glypican 3 (S359F), His Tag, low endotoxin with an affinity constant of 3.04 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
|Evaluation of CAR expression of GPC3-2246H :||
293 cells were transfected with anti-GPC3-scFv and RFP tag. 2e5 of the cells were first stained with B. Human Glypican 3 (S359F), His Tag, low endotoxin and C. His tag protein control, followed by FITC-conjugated anti-6xHis tag antibody. A. Non-transfected 293 cells and C. His tag protein control were used as negative control. RFP was used to evaluate CAR (anti-GPC3-scFv) expression and FITC was used to evaluate the binding activity of Human Glypican 3 (S359F), His Tag, low endotoxin (Cat. No. GP3-H5223).
|Gene Name :||GPC3|
|Official Symbol :||GPC3|
|Synonyms :||GPC3; glypican 3; SDYS; glypican-3; DGSX; glypican proteoglycan 3; OCI 5; SGB; SGBS; SGBS1; secreted glypican-3; intestinal protein OCI-5; heparan sulphate proteoglycan; MXR7; OCI-5; GTR2-2|
|Gene ID :||2719|
|mRNA Refseq :||NM_001164617|
|Protein Refseq :||NP_001158089|
|UniProt ID :||P51654|
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