"GPC3" Related Products

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Recombinant Human GPC3, His tagged

Cat.No.: GPC3-2246H
Product Overview: Recombinant Human GPC3 (P51654-1) (Met 1-His 559), fused with a C-terminal polyhistidine tag, was produced in Human Cell.
Source: Human Cells
Species: Human
Tag: His
Form: Lyophilized from sterile PBS, pH 7.4
Molecular Mass: The secreted recombinant human GPC3 comprises 546 amino acids and has a predicted molecular mass of 62 kDa. GPC3 is composed of proteolytic cleaved two subunits linked by disulfide bonds, that are produced by proprotein convertases. As a result of glycosylation, apparent molecular mass of rhGPC3 is approximately 40 and 50-60 kDa in SDS-PAGE under reducing conditions, corresponding to the N-terminal and C-terminal fragment respectively.
Endotoxin: < 1.0 eu per μg of the protein as determined by the LAL method.
Stability: Samples are stable for up to twelve months from date of receipt at -70oC.
Storage: Store it under sterile conditions at -20oC~-70oC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4oC before opening to recover the entire contents.
Gene Name: GPC3 glypican 3 [ Homo sapiens ]
Official Symbol: GPC3
Gene ID: 2719
mRNA Refseq: NM_001164617
Protein Refseq: NP_001158089
MIM: 300037
UniProt ID: P51654

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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