Recombinant Human GPC3 protein (S359F), His-tagged, low endotoxin GPC3-2246H

Recombinant Human GPC3 protein (S359F), His-tagged, low endotoxin

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Cat.No. : GPC3-2246H Optional Service: Optional requirements on this protein
Product Overview : Recombinant Human GPC3 (S359F, Gln25-His559)(low endotoxin) protein was fused to His-tag at C-terminus and expressed in human 293 cells (HEK293).
Description : Glypican-3 (GPC3) is also known as Intestinal protein OCI-5, GTR2-2, MXR7, which belongs to the glypican family. Glypican 3/GPC-3 is highly expressed in lung, liver and kidney. Glypican-3 inhibits the dipeptidyl peptidase activity of DPP4. Glypican-3 may be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs, and also may play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function.
Source : HEK293
Species : Human
Tag : His
Predicted N Terminal : Gln25
Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
Molecular Mass : The protein has a calculated MW of 61.7 kDa. The protein migrates as 66-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein length : Gln25-His559
Endotoxin : Less than 0.1 EU per μg by the LAL method.
Purity : >90% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE of GPC3-2246H :

Human Glypican 3 (S359F), His Tag, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA of GPC3-2246H :

Immobilized Human FGF basic, Tag Free at 0.5 μg/mL (100 μL/well) can bind Human Glypican 3 (S359F), His Tag, low endotoxin with a linear range of 3-128 ng/mL (QC tested).

Bioactivity-ELISA :

Immobilized Human Glypican 3 (S359F), His Tag, low endotoxin at 0.5 μg/mL (100 μL/well) can bind Anti-Human GPC3 Mab with a linear range of 0.4-3 ng/mL (Routinely tested).

Bioactivity-SPR of GPC3-2246H :

Anti-Human GPC3 MAb (chimeric mouse-human IgG1) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human Glypican 3 (S359F), His Tag, low endotoxin with an affinity constant of 1.96 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

Bioactivity-BLI of GPC3-2246H :

Loaded Anti-Human GPC3 MAb (chimeric mouse-human IgG1) on AHC Biosensor, can bind Human Glypican 3 (S359F), His Tag, low endotoxin with an affinity constant of 3.04 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Evaluation of CAR expression of GPC3-2246H :

293 cells were transfected with anti-GPC3-scFv and RFP tag. 2e5 of the cells were first stained with B. Human Glypican 3 (S359F), His Tag, low endotoxin and C. His tag protein control, followed by FITC-conjugated anti-6xHis tag antibody. A. Non-transfected 293 cells and C. His tag protein control were used as negative control. RFP was used to evaluate CAR (anti-GPC3-scFv) expression and FITC was used to evaluate the binding activity of Human Glypican 3 (S359F), His Tag, low endotoxin (Cat. No. GP3-H5223).

Gene Name : GPC3
Official Symbol : GPC3
Synonyms : GPC3; glypican 3; SDYS; glypican-3; DGSX; glypican proteoglycan 3; OCI 5; SGB; SGBS; SGBS1; secreted glypican-3; intestinal protein OCI-5; heparan sulphate proteoglycan; MXR7; OCI-5; GTR2-2
Gene ID : 2719
mRNA Refseq : NM_001164617
Protein Refseq : NP_001158089
MIM : 300037
UniProt ID : P51654
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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