Recombinant Human Thioredoxin, His-tagged

• Cat.No.: TXN-2356H
• Product Overview: Recombinant human TXN protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography techniques, 13.9 kDa (125aa).

Specification

• Cat. No.: TXN-2356H
• Description: Thioredoxin(Trx) is a low molecular weight redox protein. Trx contains a redox active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site. It is involved in the first unique step in DNA synthesis. Trx also provides control over a number of transcription factors affecting cell proliferation and death through a mechanism referred to as redox regulation.
• Source: E.coli.
• Form: Liquid. In Phosphate Buffered Saline pH7.4.
• Purity: > 95% by SDS – PAGE.
• Concentration: 1 mg/ml (determined by Bradford assay).
• Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH MVKQIESKTA FQEALDAAGD KLVVVDFSAT WCGPCKMIKP FFHSLSEKYS NVIFLEVDVD DCQDVASECE VKCMPTFQFF KKGQKVGEFS GANKEKLEAT INELV.
• Biological activity: Specific activity is 7-10 A650/min/mg and was obtained by measuring the increase of insulin precipitation in absorbance at 650 nm resulting from the reduction of insulin. Please refer to our activity assay protocol.
• Activity Assay: 1. Prepare a 1ml reaction cocktail into a suitable container: The final concentrations are 5mM Tris, 63mM sodium phosphate, 1mM DTT, 0.1% (w/v) insulin, 2mM EDTA. 2. Add 4ug of recombinant His-TXN into reaction cocktail and mix immediately. 3. Incubate at 25C for 20 minutes. 4. Record the increase in A650nm for 15 minutes.
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: TXN thioredoxin [ Homo sapiens ]
• Synonyms: TXN; MGC61975; TRX; ADF; DKFZp686B1993; MGC61975; OTTHUMP00000021892; SASP; TRDX; TRX1; Trx; thioredoxin; ATL-derived factor; Surface-associated sulphydryl protein
• Gene ID: 7295
• mRNA Refseq: NM_003329
• Protein Refseq: NP_003320
• MIM: 187700
• UniProt ID: P10599
• Chromosome Location: 9q31
• Pathway: Nucleotide metabolism
• Function: protein binding