"TXN" Related Products

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Recombinant Human Thioredoxin, His-tagged

Cat. No. : TXN-2356H
Product Overview : Recombinant human TXN protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography techniques, 13.9 kDa (125aa).
Description : Thioredoxin(Trx) is a low molecular weight redox protein. Trx contains a redox active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site. It is involved in the first unique step in DNA synthesis. Trx also provides control over a number of transcription factors affecting cell proliferation and death through a mechanism referred to as redox regulation.
Source : E.coli.
Form : Liquid. In Phosphate Buffered Saline pH7.4.
Purity : > 95% by SDS – PAGE.
Concentration : 1 mg/ml (determined by Bradford assay).
Sequences Of Amino Acids : MGSSHHHHHH SSGLVPRGSH MVKQIESKTA FQEALDAAGD KLVVVDFSAT WCGPCKMIKP FFHSLSEKYS NVIFLEVDVD DCQDVASECE VKCMPTFQFF KKGQKVGEFS GANKEKLEAT INELV.
Biological activity : Specific activity is 7-10 A650/min/mg and was obtained by measuring the increase of insulin precipitation in absorbance at 650 nm resulting from the reduction of insulin. Please refer to our activity assay protocol.
Activity Assay : 1. Prepare a 1ml reaction cocktail into a suitable container: The final concentrations are 5mM Tris, 63mM sodium phosphate, 1mM DTT, 0.1% (w/v) insulin, 2mM EDTA. 2. Add 4ug of recombinant His-TXN into reaction cocktail and mix immediately. 3. Incubate at 25C for 20 minutes. 4. Record the increase in A650nm for 15 minutes.
Storage : Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Gene Name : TXN thioredoxin [ Homo sapiens ]
Synonyms : TXN; MGC61975; TRX; ADF; DKFZp686B1993; MGC61975; OTTHUMP00000021892; SASP; TRDX; TRX1; Trx; thioredoxin; ATL-derived factor; Surface-associated sulphydryl protein
Gene ID : 7295
mRNA Refseq : NM_003329
Protein Refseq : NP_003320
MIM : 187700
UniProt ID : P10599
Chromosome Location : 9q31
Pathway : Nucleotide metabolism
Function : protein binding

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