Recombinant Mouse Cd274 protein, His-tagged Cd274-2275M

Recombinant Mouse Cd274 protein, His-tagged

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Cat.No. : Cd274-2275M Optional Service: Optional requirements on this protein
Product Overview : Recombinant Mouse Cd274 (Phe19-Thr238) protein was fused to His-tag at C-terminus and expressed in human 293 cells (HEK293).
Description : Programmed cell death 1 ligand 1 (PD-L1) is also known as cluster of differentiation (CD274) or B7 homolog 1 (B7-H1), is a member of the growing B7 family of immune molecules and is involved in the regulation of cellular and humoral immune responses. B7-H1 is a cell surface immunoglobulin superfamily with two Ig-like domains within the extracellular region and a short cytoplasmic domain. PD-L1 is highly expressed in the heart, skeletal muscle, placenta and lung and weakly expressed in the thymus, spleen, kidney and liver. PD-L1 is expressed on activated T-cells, B-cells, dendritic cells, keratinocytes and monocytes. PD-L1 is up-regulated on T- and B-cells, dendritic cells, keratinocytes and monocytes after LPS and IFNG activation and up-regulated in B-cells activated by surface Ig cross-linking. PD-L1 involve in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner.
Source : HEK293
Species : Mouse
Tag : His
Predicted N Terminal : Phe19
Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
Molecular Mass : The protein has a calculated MW of 26.6 kDa. The protein migrates as 40-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein length : Phe19-Thr238
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE of Cd274-2275M :

Mouse PD-L1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-SPR of Cd274-2275M :

Captured Mouse PD-1, Fc Tag on Protein A Chip can bind Mouse PD-L1, His Tag with an affinity constant of 2.16 μM as determined in SPR assay (Biacore T200) (Routinely tested).

Bioactivity-SPR :

Captured Mouse PD-L1, His Tag on CM5 Chip via Anti-His antibody can bind Mouse PD-1, Fc Tag with an affinity constant of 1.37 μM as determined in SPR assay (Biacore T200) (Routinely tested).

Bioactivity-BLI of Cd274-2275M :

Loaded Mouse PD-1, Fc Tag on ProteinA Biosensor, can bind Mouse PD-L1, His Tag with an affinity constant of 1.5 μM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).

Batch consistency of Bioactivity-BLI

Gene Name : Cd274
Official Symbol : Cd274
Synonyms : CD274; CD274 antigen; programmed cell death 1 ligand 1; B7 homolog 1; PDCD1 ligand 1; programmed death ligand 1; B7h1; Pdl1; Pdcd1l1; Pdcd1lg1; A530045L16Rik
Gene ID : 60533
mRNA Refseq : NM_021893
Protein Refseq : NP_068693
UniProt ID : Q9EP73
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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